한국어병학회지 (Journal of fish pathology)

  • 제16권2호
  • /
  • Pages.81-90
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  • 2003
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

무지개송어 성어에 대량 폐사를 유발하는 IHNV의 분리와 특성

Isolation and Characterization of Infectious Hematopoietic Necrosis Virus Causing High Mortality in Rainbow Trout Oncorhynchus mykiss

  • 김기홍 (여수대학교 수산생명의학과) ;
  • 김영진 (여수대학교 수산생명의학과) ;
  • 정성주 (여수대학교 수산생명의학과) ;
  • 정태성 (경상대학교 수의학과) ;
  • 오명주 (여수대학교 수산생명의학과)
  • Kim, Gi-Hong (Department of Aqualife Medicine. Yosu National University) ;
  • Kim, Yeong-Jin (Department of Aqualife Medicine. Yosu National University) ;
  • Jeong, Seong-Ju (Department of Aqualife Medicine. Yosu National University) ;
  • Jeong, Tae-Seong (Lab of Fish & Shellfish Diseases, KyungSang National University) ;
  • O, Myeong-Ju (Department of Aqualife Medicine. Yosu National University)
  • 발행 : 20030000
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초록

High mortality with signs similar to viral haemorrhagic septicemia (VHS) such as severe haemorrhages in the skin, muscle and air bladder occurred in the farmed adult rainbow trout, Oncorhynchus mykiss, in Ku-mi and Je-chun area in Korea. The isolates were neutralized by an antiserum against IHNV but not by antisera against VHSV. Electron micrograph of an ultrathin section showed large numbers of bullet-shaped virus particles. The newly isolated rhabdovirus was composed of five structural proteins. In the western blot analysis Ihe anti-DiNV serum strongly reacted with G. N and MI protein. The cumulative mortalities of RTK infected rainbow trout (10-12cm.9-12g) with $10^{3.5}\;and\;10^{1.5}TCID_{50}/m{\ell}$ were 80% and 30%. respectiveIy_ RTJ infected fish showed 50% mortality by infection with $10^{3.5}TCID_{50}/m{\ell}$. Control group and IHNVChAb exhibited no mortality. From these results, the viruses were identified lHNV although diseased fish showed similar sign. with VHS and caused high mortality in large-sized fish.

키워드

참고문헌

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