잉어류 바이러스성전신괴사증바이러스 (VSNCV) 백신 투여에 대한 잉어의 면역반응

Immune responses th the vaccines of viral systemic necrosis of carp virus (VSNCV) of comom carp, Cyprinus carpio L.

  • 발행 : 20031200

초록

양식 잉어류에서 심각한 피해를 야기하고 있는 VSNCV에 대한 백신을 제작하여 잉어에 투여한 후 특이적 및 비특이적 면역반응을 조사하였다. 백신은 포르마린처리백신 (FKV)과 가온처리백신 (HKV)를 제작하여 건강한 잉어에 0.2㎖씩 복강주사 하였으며, 2주 후 동일한 방법으로 boost 처리하였다. 백신 투여 후 비특이적 면역반응 중 혈청 라이소자임 활성과 대식세포의 chemiluminescent (CL) reponses은 1차 주사 후 및 boost 처리 후 2일째부터 7일째까지 유의적인 증가를 나타내었으며, 이후 대조구 수준으로 감소하였다. ELISA법으로 항체가를 조사한 결과 대조구를 제외한 FKV와 HKV 투여구에서 주사 후 2주 째부터 boost 처리 후 6주 째까지 유의적으로 증가하였으며, 그 중에서도 boost 처리 후 2주 째 최고치를 나타내었다. 각 시험구별로 boost 처리 후 2주 째에 VSNCV로 공격 실험한 결과 FKV 투여구에서는 20%의 누적폐사율을 나타내어 방어력이 인정되었으나, HKV 투여구에서는 70%의 누적폐사율이 나타나 방어효과가 거의 없는 것으로 나타났다.

VSNC is a viral disease causing significant economic losses in cultured carp Ciprinus carpio L. in Korea. Carps were immunized with prepared vaccines against VSNCV and examined specific and nonspecific immune responses. Carps were injected by O.2㎖ of formalin-killed vaccine (FKV), heat-killed vaccine (HKV) or E-MEM, respectively and dealt with boost with same way two weeks later. The lysozyme activity of serum and chemiluminescent reponses of head-kidney leucocytes showed increased responses during 2-7 days post-first injection (pfi) and post-boost (pb) in the vaccinated fish, and then decreased to the level of control. As measured by ELISA, vaccinated groups showed a significant increase in VSNCV-specific serum antibodies between 2 weeks pfi and 6weeks pb with a peak at 2 weeks pb. Results of the virus challenge showed that the fish vaccinated with FKV have induced protective immunity, while HKV injection hardly provided protection.

키워드

참고문헌

  1. 노용길, 이배익, 김광석, 강언종, 김이청, 손재경 : 내수면 양식의 개요. pp.3-7. 진해내수면연구소, 1997
  2. Agneil, L. D. : An assessment of passive transfer of immunity to infectious pancreatic necrosis virus (IPNV) in trout. M. S. thesis. American University, Washington, D. C., 1975
  3. Alexander, J. B. and Ingram. G. A. : Noncellular nonspecific defence mechanisms of fish. Ann. Rev. Fish Dis., 2:249-280, 1992 https://doi.org/10.1016/0959-8030(92)90066-7
  4. Bootland, L. M., Dobos, P. and Stevenson, R. M. W. : Immunization of adult brook trout, Salvelinus fontinalis (Mitchell), fail to prevent the infectious pancreatic necrosis virus (IPNV) carrier state. J. Fish Dis. 18:449-458,1995 https://doi.org/10.1111/j.1365-2761.1995.tb00336.x
  5. Buchanan, K. L. and Murphy, J. W. : Characterization of cellular infiltrates and cytokine production during the expression phase of the anticrytococcal delayed-type hypersensitivity response. Infec. Immun.61:2854-2865,1993
  6. Desvignes, L., Quentel, C., Lamour, F. and LE Ven. A. : Pathogenesis and immune response in Atlantic salmon (Salmo salar L.) parr experimentally infected with salmon pancreas disease virus (SPDV). Fish & Shellfish Immunol., 12:77-95, 2002 https://doi.org/10.1006/fsim.2001.0356
  7. Ellis, A. E. : Lysozyme assays. In 'Techniques in fish immunology'. Stolen, J. S. Flecher, T.C., Anderson, D.P., Roberson, B.S. and van Muiswinkel, W.B., eds., pp. 101-103, Fair Haven, NJ:SOS Publication, 1990
  8. Ellis, A. E. : Innate host defense mechanisms of fish against viruses and bacteria. Dev. Comp. Immunol.,25:827-839, 2001 https://doi.org/10.1016/S0145-305X(01)00038-6
  9. Fletcher, T. C. and White, A. : The lysozyme of the plaice Pleuronecyes platessa L. Comp. Biochem. Physiol., 55:207-210, 1976 https://doi.org/10.1016/0300-9629(76)90132-8
  10. Jolles, P. and Jolles, J. : What's new in lysozyme research? Always model system, today as yesterday. Mol. Biochem., 63:165-189, 1984
  11. Khakpour, F. R. and Murphy, J. W. : Characterization of a third-order suppressor T cell(Ts3) induced by crypococcal antigen(s). Infec. Immun., 55:1657-1662, 1987
  12. Murphy, J. W., Schafer, F., Casadevall, A. and Adesina, A. : Antigen-induced protective nonprotective cell-mediated immune components against Crytococcus neoformans. Infec. Immun., 66(6):2632-2639, 1998
  13. Oh, M.J., Jung, S. J., Choi, T. J., Kim, H. R., Rajendran, K. V., Kim, Y. J., Park, M. A. and Chun., S. K. : A viral disease occurring in cultured carp in Korea. Fish Pathol., 36:147-151, 2001 https://doi.org/10.3147/jsfp.36.147
  14. Paulsen, S. M., Lunde, H., Engstad, R. E. and Robertsen, B. : In vivo effects of $\beta$-glucan and LPS on regulation of lysozyme activity and mRNA expression in Atlantic salmon (Salmo salar L.). Fish & shellfish Immunol., 12:1-16, 2002
  15. Reno, P. W. : Qualitative and quantitative aspects of the infectious pancreatic necrosis virus (IPN) carrier state in trout. Ph. D. thesis. University of Guelph, Ontario, Canada, 1976
  16. Smail, D. A. and Munro, A. L. S. : Infectious pancreatic necrosis virus persistence in farmed Atlantic salmon (Salmo salar). In Fish and Shellfish Pathology. A. E. Ellis, ed., pp.277-288. London : Academic Press, 1985
  17. Sveinbjørnsson, B., Olsen, R. and Paulsen, S. : Immunocytochemical localization of lysozyme in intestinal eosinophilic granular cells (EGCs) of Atlatic salmon, Salmo salar L. J. Fish Dis., 19:349-355, 1996 https://doi.org/10.1111/j.1365-2761.1996.tb00373.x
  18. Yamamoto, T. : Infectious pancreatic necrosis (IPN) virus carriers and antibody production in a population of rainbow trout (Salmo gairdneri). Can. J. Microbiol. 21:1343-1347, 1975 https://doi.org/10.1139/m75-201
  19. Yoan, R., Casadevall, A., Oh, J. and Scharff, M. D. : T cells cooperate with passive antibody to modify Crytococcus neoformans infection in mice. Proc. Natl. Acad. Sci. USA, 94:2483-2488, 1997 https://doi.org/10.1073/pnas.94.6.2483