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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Suppression of Fatty Acid Synthase by Dietary Polyunsaturated Fatty Acids is Mediated by Fat itself, not by Peroxidative Mechanism

  • Kim, Hye-Kyeong (Department of Food and Nutrition, Seoul National University) ;
  • Choi, Sung-Won (Department of Food and Nutrition, Seoul National University) ;
  • Lee, Hae-Jeung (Department of Food and Nutrition, Seoul National University) ;
  • Lee, Joo-Hee (Department of Food and Nutrition, Kyungsang National University) ;
  • Choi, Hay-Mie (Department of Food and Nutrition, Seoul National University)
  • Received : 2002.10.08
  • Accepted : 2002.12.11
  • Published : 2003.05.31
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Abstract

This study examined the effect of dietary polyunsaturated fatty acids (PUFA) that were supplemented with vitamin E on lipid peroxidation, glutathione-dependent detoxifying enzyme system activity, and lipogenic fatty acid synthase (FAS) expression in rat liver. Male Sprague-Dawley rats were fed semipurified diets containing either 1% (w/w) corn oil or 10% each of beef tallow, corn oil, perilla oil, and fish oil for 4 wk. Alpha-tocopherol was supplemented in perilla oil (0.015%) and fish oil (0.019%). Hepatic thiobarbituric acid reactive substances, an estimate of lipid peroxidation, were not significantly different among the dietary groups. The glutathione peroxidase, glutathione reductase, and glutathione S-transferase activities were all elevated by the polyunsaturated fats, especially fish oil. The activity of FAS was reduced in the polyunsaturated fat-fed groups in the order of fish oil, perilla oil, and corn oil. The mRNA contents decreased in rats that were fed the 10% fat diets, particularly polyunsaturated fats, compared with the rats that were fed the 1% corn oil diet. Similarly, the inhibitory effect was the greatest in fish oil. These results suggest that lipid peroxidation can be minimized by vitamin E; PUFA in itself has a suppressive effect on lipogenic enzyme.

Keywords

References

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