Journal of Gastric Cancer
- Volume 3 Issue 4
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- Pages.186-190
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- 2003
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- 2093-582X(pISSN)
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- 2093-5641(eISSN)
Functional Defect of the Fas Mutants Detected in Gastric Cancers
위암에서 발견된 돌연변이형 Fas 단백의 기능적 결함
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Park Won Sang
(Departments of Pathology College of Medicine, The Catholic University of Korea) ;
- Cho Young Gu (Departments of Pathology College of Medicine, The Catholic University of Korea) ;
- Kim Chang Jae (Departments of Pathology College of Medicine, The Catholic University of Korea) ;
- Park Cho Hyun (Departments of Surgery, College of Medicine, The Catholic University of Korea) ;
- Kim Young Sil (Departments of Pathology College of Medicine, The Catholic University of Korea) ;
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Kim Su Young
(Departments of Pathology College of Medicine, The Catholic University of Korea) ;
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Nam Suk Woo
(Departments of Pathology College of Medicine, The Catholic University of Korea) ;
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Lee Sug Hyung
(Departments of Pathology College of Medicine, The Catholic University of Korea) ;
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Yoo Nam Jin
(Departments of Pathology College of Medicine, The Catholic University of Korea) ;
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Lee Jung Young
(Departments of Pathology College of Medicine, The Catholic University of Korea)
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박원상
(가톨릭대학교 의과대학 병리학교실) ;
- 조용구 (가톨릭대학교 의과대학 병리학교실) ;
- 김창재 (가톨릭대학교 의과대학 병리학교실) ;
- 박조현 (가톨릭대학교 의과대학 외과학교실) ;
- 김영실 (가톨릭대학교 의과대학 병리학교실) ;
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김수영
(가톨릭대학교 의과대학 병리학교실) ;
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남석우
(가톨릭대학교 의과대학 병리학교실) ;
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이석형
(가톨릭대학교 의과대학 병리학교실) ;
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유남진
(가톨릭대학교 의과대학 병리학교실) ;
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이정용
(가톨릭대학교 의과대학 병리학교실)
- Published : 2003.12.01
Abstract
Purpose: The balance between cell proliferation and apoptosis is crucial for homeostatic maintenance in a cell population. Decreased apoptosis or uncontrolled proliferation can lead to cancer. The Fas receptor signal through a cytoplasmic death domain is very important in the apoptotic pathway. To identify the effect of the death domain of the Fas gene in the development and/or progression of gastric cancer, we examined the apoptotic potential of five known Fas mutants detected in gastric cancers. Materials and Methods: A wild-type Fas gene was cloned with cDNA from normal liver tissue and full length Fas was sequenced. Mutants of the gene were generated with sitedirected mutagenesis by using the wild-type gene and specific primers. Wild- and mutant-type genes were transfected to HEK293 cells. Forty-eight hours after transfection the cells were stained with DAPI and cell death was counted under fluorescent microscopy. Results: In wild-type Fas-transfected cells, the percentage of apoptotic cells was