The korean journal of orthodontics (대한치과교정학회지)

The Korean Association Of Orthodontists (대한치과교정학회)
권호 표지

Effect of Leaf-Extract from Camellia sinensis and Seed-Extract from Casia tora on Viability of Mutans Streptococci isolated from the interface between orthodontic brackets and tooth surfaces

녹차 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

  • Lim, Sung-Hoon (Department of Orthodontcis, College of Dentisry, Chosun University) ;
  • Seo, Jeong-Soon (Department of Orthodontcis, College of Dentistry, Chosun University) ;
  • Yoon, Young-Jooh (Department of Orthodontcis, College of Dentistry, Chosun University) ;
  • Kim, Kwang-Won (Department of Orthodontcis, College of Dentistry, Chosun University) ;
  • Yoon, So Young (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Lee, Byoung-Rai (Department of Biochemistry, Medical College, Chosun University) ;
  • Cha, Jong-Hee (Department of Biochemistry, Medical College, Chosun University) ;
  • Park, Jae-Yoon (Department of Biochemistry, Medical College, Chosun University)
  • 임성훈 (조선대학교 치과대학 교정학교실) ;
  • 서정순 (조선대학교 치과대학 교정학교실) ;
  • 윤영주 (조선대학교 치과대학 교정학교실) ;
  • 김광원 (조선대학교 치과대학 교정학교실) ;
  • 유소영 (조선대학교 치과대학 구강생화학교실) ;
  • 김화숙 (조선대학교 치과대학 구강생화학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실) ;
  • 이병래 (조선대학교 의과대학 생화학교실) ;
  • 차종희 (조선대학교 의과대학 생화학교실) ;
  • 박재윤 (조선대학교 의과대학 생화학교실)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatmet. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casia torn were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea nay be more effective than the tea Prepared from C tora In the prevention of enamel decalcification or dental caries around brackets.

본 연구는 치아우식증에 관련된 Hutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 $50\%$ 에탄올을 이용하여얻었다. 이들 추출물의 Mutans streptococci표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장 억제농도는 5mg/ml이었다 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다.

Keywords

References

  1. Fitzgerald RJ, Keyes PH, Demonstration of the etologic role of streptococci in experimental caries in the hamster. JADA. 1960 : 61 : 9-19
  2. Michalek SM, McGhee JR, Shiota T, Devenyns D. Virulence of Strep- tococcus mutans: Cariogenicity of S mutans in adult gnotobiotic rats. Infect Immun 1977 : 15 : 466-71
  3. De Stoppelaar JD, van Houte J, Backer Dirks O. The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res 1969 : 3 : 190-9
  4. Krasse B. Relationship between caries activity and the number of lactobacilli in the oral cavity. Acta Odont Scand 1954 : 12: 157-72
  5. Jordan HV, Keyes PH, Bellack S. Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. J Periodont Res 1972 : 7 : 21-8
  6. Gibbons RJ, Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol 1968 : 13 : 1249-62
  7. Loesche WJ. Dental Caries: a treatable infection. Charles C Thomas, Publisher, Spring field, 1982
  8. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998 : 13 : 195-216
  9. Hallgren A, Oliveby A, Twetrnan S. Caries associated microflora in plaque from orthodontic appliances retained with glass ionomer cement. J Dent Res 1992 : 100: 140-3
  10. Sukontapatipark W, el-Agroudi MA, Selliseth NJ, Thunold K, Selvig K. Bacterial colonization associated with fixed orthodontic appliances. Eur J Orthod 2001 : 23 : 475-84
  11. Artun J, Brobakken BO. Prevalence of carious white spots after orthodontic treatment with multibonded appliances. Europ J Orthod 1986 : 8 : 229-34
  12. Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding. Am J Orthod 1982 : 81 : 93-8
  13. Murray PR, Jorgensen JH. Quantitative susceptibility test methods in major united states medical center. Antimicrob Agents Chemother 1981 : 20 : 66-70
  14. Tsuchiya H. Effects of green tea catechins on membrane fluidity. Pharmacology 1999 : 59 : 34-44
  15. Hamada S, Kontani M, Hosono H, et. al. Peroxidase-catalyzed generation of catechin oligomers that inhibit glucosyltransferase from Streptococcus sobrinus. FEMS Microbiol Lett 1996 : 143: 35-40
  16. Hattori M, Kusumoto IT, Namba T, Ishigami T, Hara Y. Effect of tea polyphenols on glucan synthesis by glucosyltransferase from Streptococcus mutans. Chem Pharm Bull (Tokyo) 1990 : 38 : 717-20
  17. Ooshima T, Minami T, Matsumoto M, Fujiwara T, Sobue S, Hamada S. Comparison of the cariostatic effects between regimens to administer oolong tea polyphenols in SPF rats. Caries Res 1998 : 32 : 75-80
  18. Sarkar S, Sett P, Chowdhury T, Ganguly DK. Effect of black tea on teeth. J Indian Soc Pedod Prev Dent. 2000 : 18: 139-40
  19. Yanagida A, Kanda T, Tanabe M, Matsudaira F, Oliveira Cordeiro JG. Inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci. J Agric Food Chem 2000 : 48 : 5666-71
  20. Kitamura K, Loyola JP, Sobue S. Inhibitory effects of a hot water extract from Japanese tea on the cell growth of mutans streptococci. Shoni Shikagaku Zasshi 1990 : 28 : 618-22
  21. Matsumoto M, Minami T, Sasaki H. et. al. Inhibitory effects of oolong tea extract on caries-inducing properties of mutans streptococci. Caries Res 1999 : 33 : 441-5