Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of the High-Glutathione Producing Saccharomyces cerevisiae FF-8 from Korean Traditional Rice Wine and Optimal Producing Conditions

전통 발효주로부터 glutathione 고함유 효모 Saccharomyces cerevisiae FF-8의 분리.동정 및 최적 생산조건

  • Park, Jin-Chul (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Ok, Min (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Cha, Jae-Young (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
  • 박진철 (동아대학교 응용생명공학부 생명공학과) ;
  • 옥민 (동아대학교 응용생명공학부 생명공학과) ;
  • 차재영 (동아대학교 응용생명공학부 생명공학과) ;
  • 조영수 (동아대학교 응용생명공학부 생명공학과)
  • Published : 2003.11.30
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Abstract

In this study, strain of high-producing intracellular glutathione was isolated from Korean traditional rice wine. The isolated strain was identified as Saccharomyces cerevisiae based on the morphological, physiological and biochemical characteristics, and was designated as FF-8. The optimal condition for glutathione production by Saccharomyces cerevisiae FF-8 was obtained after cultivation with shaking for 72 hours in the YM medium. The optimal temperature, shaking rate and initial pH for the glutathione production were $30^{\circ}C$, 100 rpm and pH 6.0, respectively. The dry cell weight and glutathione concentration produced by Saccharomyces cerevisiae FF-8 were 5.2 g/l and 72.0 mg/l, respectively, under the optimal culture condition.

본 연구에서는 $glutathione({\gamma}$-L-glutamyl-cysteinyl-glycine)을 다량으로 함유하는 효모 균주를 전통 발효주로부터 분리하여 glutathione 생산 조건을 검토하였다. 분리된 균주는 형태학적, 생리학적 및 생화학적 특성을 검토한 결과 Saccharomyces cerevisiae로 동정되어 FF-8로 명명하였다. Saccharomyces cerevisiae FF-8의 glutathione 생산 조건은 YM(glucose 1.0%, acid peptone 0.5%, yeast extract 0.3%, malt extract 0.3%) 배지를 기본으로 하여 최적 온도, 교반속도 및 초기 pH의 조건을 검토하였다. Glutathione 생산은 온도 $30^{\circ}C$, 교반속도 100 rpm 및 pH 6.0에서 72시간 동안 진탕 배양하였을 때 가장 높았으며, 이때 glutathione 생산량은 72.0 mg/l이였으며, 건조 균체량은 5.2 g/l이였다.

Keywords

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