Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidantive, Phospholipase $A_2$ Inhibiting, and Anticancer Effect of Polyphenol Rich Fractions from Panax ginseng C. A. Meyer

한국산 인삼의 Polyphenol 분획물의 항산화, Phospholipase $A_2$ 및 암세포증식 억제효과

  • Choi, Hee-Jin (Department of Food Science & Technology College of Natural resources Yeungnam University) ;
  • Han, Ho-Suk (Department of Food Science & Technology College of Natural resources Yeungnam University) ;
  • Park, Jung-Hye (Department of Food Science & Technology College of Natural resources Yeungnam University) ;
  • Son, Jun-Ho (Korea Advanced Food Research Institute) ;
  • Bae, Jong-Ho (Department of Confectionery Decoration, Daegue Mirae College) ;
  • Seung, Tae-Su (Department of Food & Nutrition, Changwon Junior College) ;
  • Choi, Cheong (Department of Food Science & Technology College of Natural resources Yeungnam University)
  • 최희진 (영남대학교 생물산업공학부) ;
  • 한호석 (영남대학교 생물산업공학부) ;
  • 박정혜 (영남대학교 생물산업공학부) ;
  • 손준호 (한국식품연구소) ;
  • 배종호 (대구미래대학 제과데코레이션과) ;
  • 성태수 (창원전문대학 식품영양과) ;
  • 최청 (영남대학교 생물산업공학부)
  • Published : 2003.08.30
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Abstract

The polyphenol fractions of Korean ginseng were purified using Sephadex LH-20, MCI gel, Bondapak $C_{18}$ TLC, and HPLC from the 60% acetone soluble fraction. Fraction I showed 48.16%, 79.71% and 43.55% inhibition at 150 ppm against lipid oxidation in the presence of copper ion, superoxide and hydrogen peroxidation. Electron donating abilities of fraction II showed 35.17% inhibition at 200 ppm. Fraction III showed 48.49% and 25% inhibition at 150 ppm against lipid oxidation in the presence of ferrous ion and hydroxy radical ion. The phospholipase $A_2$ inhibitory effect of fraction III was 48.9% at the concentration of $60\;{\mu}g/ml$. The cytotoxic effects of fraction II was the highest (73.29% at 0.25 mg/ml) among the tested polyphenol fractions.

한국산 인삼을 60% acetone으로 추출하여 Sepadex LH-20 gel column chromatography, MCI-CHP 20 gel column chromatography, ${\mu}-Bondapack\;C_{18}$ gel column chromatography을 이용하여 polyphenol 분획물을 3종류를 분리하여 항산화 효과, phospholipase $A_2$ 저해효과 및 암세포 증식억제 효과를 검토하였다. 항산화 효과에서 분획물 I은 150 ppm에서 $Cu^{2+},\;KO_2$ 그리고 $H_2O_2$에 대하여 각각 48.16%, 79.71%, 43.55%를 나타내었고 DPPH 수소공여능은 분획물 II가 200 ppm에서 35.17%의 라디칼소거능을 보였다. 분획물 III는 $Fe^{2+}$, OH가 존재 시 150 ppm에서 48.49%, 25%의 항산화효과를 나타내었다. Phospholipase $A_2$ 저해활성은 분획물 III이 $60\;{\mu}/ml$에서 48.9%의 활성을 나타냈다. HT 29 cell에 대한 인삼의 세포 독성은 분획물 II에서 0.25 mg/ml에서 73.29%의 가장 높은 억제능을 나타내었다.

Keywords

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