Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Rapid Detection of Salmonella Species in Foods Using PCR

PCR을 이용한 식품 내 Salmonella 균주의 신속 검출방법

  • 정상훈 (경희대학교 생명과학대학) ;
  • 김묘영 (경희대학교 생명과학대학) ;
  • 김현중 (경희대학교 생명과학대학) ;
  • 김태운 (경희대학교 생명과학대학) ;
  • 유상렬 (서울대학교 식품공학과) ;
  • 김해영 (경희대학교 생명과학대학)
  • Published : 2003.08.30
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Abstract

This study was carried out to investigate the simple and rapid detection of Salmonella species in different kinds of food using PCR method. The specific primer sets (SIN1 and SIN2) was designed and utilized to amplify a 617 bp DNA fragment from salmonella species. The sensitivity of PCR was 1 pg of purified template DNA or $10^2$ cells from pure culture. The detection limit of Salmonella typhimurium on agarose gel electrophoresis was $10^3{\sim}10^4$ cells/g in the artificially contaminated food samples. These results suggested that this simple method could be applied to industrial fields for detection of Salmonella species in food.

여러 종류의 식품에서 Salmonella 균주를 손쉽고 빠르게 검출하기 위하여, Salmonella 장독소 유전자(stn)를 기초로 제작한 primer (STN1, STN2)를 이용하여 PCR을 수행한 결과 617 hp의 특이적인 DNA단편을 얻을 수 있었다. PCR 민감도는 순수 배양한 균체에서 추출한 template DNA는 1 pg까지 검출이 가능하였고, 직접 균체를 template로 이용한 경우에는 $10^2\;cells$까지 검출이 가능하였다. Salmonella typhimurium을 인위적으로 접종시킨 식품에서는 식품 1 g당 $10^3{\sim}10^4$ cells까지 검출할 수 있었다. 이러한 결과는 PCR을 이용하여 Salmonella에 오염된 식품에서 이들 균주를 간편하고 신속하게 검출할 수 있을 것이다.

Keywords

References

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