Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidative Activity and Produced Condition of Antioxidative Substance by Bacillus sp. FF-7

Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성

  • Cha, Jae-Young (Department of Biotechnology, Dong-A University) ;
  • Kim, Hyo-Jung (Department of Biotrchnology, Dong-A University) ;
  • Jun, Bang-Sil (Department of Biotrchnology, Dong-A University) ;
  • Park, Jin-Chul (Department of Biotrchnology, Dong-A University) ;
  • Ok, Min (Department of Biotrchnology, Dong-A University) ;
  • Cho, Young-Su (Department of Biotrchnology, Dong-A University)
  • 차재영 (동아대학교 응용생명공학부) ;
  • 김효정 (동아대학교 응용생명공학부) ;
  • 전방실 (동아대학교 응용생명공학부) ;
  • 박진철 (동아대학교 응용생명공학부) ;
  • 옥민 (동아대학교 응용생명공학부) ;
  • 조영수 (동아대학교 응용생명공학부)
  • Published : 2003.08.30
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Abstract

The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by $DPPH\;({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$ free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 16S rRNA sequence, and named FF-7. The most optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and l% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peruxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free malondialdehyde production. The antioxidative activity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by IBA method was effectively inhibited during four days, and 0.05% BHT (butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carton source and l% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vit개 system and in linoleic acid peroxidation.

발효식품 시료로부터 분리된 세균을 $DPPH({\alpha},{\alpha}'-diphenyl-{\beta}-picrylhydrazyl)$ 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 165 rRNA 염기서열을 조사한결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%) >심장(79.95%) >신장(77.84%) >비장(77.47%) >고환(69.96%) >간장(62.45%) 순이였다. Linoleic acid의 과산화지질를 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다.

Keywords

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