Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Terpenoids from the Flower of Rhododendron yedoense var. poukhanense

산철쭉(Rhododendron yedoense var. poukhanense) 꽃으로부터 Terpenoid의 분리.동정

  • Hong, Yoon-Hee (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Song, Myoung-Chong (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Han, Jae-Taek (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Jang, Tae-O (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Lee, Youn-Hyung (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Seung-Ae (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Park, Mi-Hyun (Eromlife Co. Ltd.) ;
  • Baek, Nam-Ln (Graduate School of Biotechnology and Plant Metabolism Research Center, Kyung Hee University)
  • 홍윤희 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 한재택 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 장태오 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 이윤형 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 김승애 (경희대학교 동서의학대학원) ;
  • 박미현 ((주)이롬라이프) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2003.05.31
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Abstract

Extracts were obtained from the flower of Rhododendro yedoense var. poukhanense. (7 kg) in 80% aqueous MeOH and successively fractionated with solvent of EtOAc, n-BuOH and $H_2O$, successively. Silica gel and ODS column chromatographies of the EtOAc and n-BuOH fractions were repeatedly carried out by using the various solvent systems to give five terpenoids. Chemical structures of the isolated terpenoids were determined as $2{\alpha},3{\beta}-dihydroxylolean-12-ene$ (1), ursolic acid (2), grayanotoxin IV (3), grayanotoxin I (4) and grayanotoxin III (5) based on the interpretation of several spectral data including 2D-NMR such as $^1H-^1H\;COSY$, HMQC and HMBC.

산철쭉 끝을 MeOH로 추출하고, 이를 EtOAc, n-BuOH과 $H_2O$로 분배 추출하였다. EtOAc추출물과 n-BuOH 추출물에 대하여 silica gel과 ODS column을 이용하여 chromatography하거나, 아세틸화 한 후 sillica gel column chromatography를 수행하여 2종의 triterfenoid와 3종의 diterpenoio를 분리하였다. 각 화합물은 $^1H-^lH\;COSY$, HMQC, HMBC와 같은 2D-NMR기법을 포함한 스펙트럼 데이터를 해석하여 $2{\alpha}\;3\{beta}-dihydroxylolean-12-ene (1), ursolic acid (2), grayanotoxin IV (3), grayanotoxin I (4) 및 grayanotoxin III (5) 등으로 동정하였다.

Keywords

References

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