Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Separation of Heme-iron by Dialysis

투석법에 의한 Heme-iron의 분리

  • Kang, In-Kyu (Department of Food Science and Technology, and Food Science Research Institute, Kongiu National University) ;
  • In, Man-Jin (Department of Human Nutrition and Food Science, Chungwoon University) ;
  • Oh, Nam-Soon (Department of Food Science and Technology, and Food Science Research Institute, Kongiu National University)
  • 강인규 (공주대학교 식품공학과 및 공주대학교 식품과학연구소) ;
  • 인만진 (청운대학교 식품영양학과) ;
  • 오남순 (공주대학교 식품공학과 및 공주대학교 식품과학연구소)
  • Published : 2003.05.31
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Abstract

A method for separating heme-iron from hemoglobin (Hgb) hydrolysate by dialysis was developed. Recovery of heme-iron increased with increasing Hgb concentration, whereas rejection of peptide and separation effciency expressed by HP ratio (heme-iron/peptide) did not show significant differences. HP ratio increased with increases in the degree of hydrolysis of Hgb and $KH_2PO_4$ concentrations of dialysis solution. Recovery of heme-iron decreased with increase in the pH of dialysis solution due to wash-out of heme-iron across the dialysis membrane caused by increase in solubility of heme-iron. Rejections of peptide were 74.5 and 87.5% (2 and 5 kDa of cut off size, respectively), whereas recovery of heme-iron decreased from 86.5 (2 kDa) to 63.1% (25 kDa). Amounts of heme-iron and peptide of dried heme-iron product were 21.7 and 77.0%, and HP ratio and production yield were 28.2 and 6.5%, respectively.

Hemoglobin(Hgb)의 가수분해물로부터 heme-iron을 분리하는데 영향을 미치는 Hgb의 농도, 가수분해도(DH, degree of hydrolysis), 투석용액의 pH, 투석막의 pore size에 대하여 조사하였다. 가수분해에 사용된 Hgb의 농도가 높아짐에 따라 heme-iron의 회수율은 증가하였으나, peptide의 제거율과 HP ratio(총 peptide 중 heme-iron이 차지하는 비율)는 Hgb의 농도에 따른 큰 차이를 볼 수 없었다. Hgb의 가수분해도가 8%, 16%, 24%로 증가함에 따라 투석에 의한 peptide의 제거가 용이하게 이루어져 HP ratio가 13.7%, 20.7%, 31%로 증가하였다. 투석용액에 $KH_2PO_4$를 25 mM이 되도록 첨가할 때는 HP ratio가 25.7%이었으나,50 mM이상의 농도에서는 30.0-32.5%로 증가하였다. 투석용액의 pH 높을수록 heme-iron의 용해도가 증가하여 투석막을 통한 heme-iron의 세출이 많아졌다. Peptide의 제거율은 투석막이 2kDa에서 5kDa로 커질 때 74.5%에서 87.5%의 큰 폭으로 증가하였으며, heme-iron의 회수율은 투석막의 크기에 따라 감소하여 2kDa에서는 86.5%, 15kDa에서는 79.6%, 25kDa에서 63.1%로 급감하였다. 투석법에 의해 분리, 건조된 heme-iron제품의 heme-iron과 peptide의 함량은 각각 21.7%와 77.0%이었으며, HP ratio는 28.2%, 수율은 6,5%이었다.

Keywords

References

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