거대배 돌연변이 계통벼 쌀 배유 전분의 이화학적 특성

Some Physical Properties of Starch Granules from Giant Embryonic Rice Endosperm

  • Kang, Mi-Young (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Lee, Yun-Ri (Department of Food Science and Nutrition, Kyungpook National University) ;
  • Koh, Hee-Jong (College of Agricultural and life Science, Seoul National University) ;
  • Nam, Seok-Hyun (Department of Biological Science, Ajou University)
  • 발행 : 2003.05.31

초록

화청찰벼, 화청벼, 그리고 이들의 거대배아미 품종인 화청찰거대배아미, 화청거대배아미 등 4품종의 쌀로부터 전분을 분리하여 몇몇 이화학적 특성을 비교하였다. 거대배아 품종으로 유기되면서 아밀로오스의 함량은 감소하고 있었으며, 아밀로오스 분획의 포도당 사슬의 길이도 짧아지고 있었다. 아밀로펙틴의 미세구조는 거대배아 품종으로 유기됨에 따라 B chain의 사슬길이가 약간씩 증가하는 경향이 있고, A chain의 양은 감소하고 있었으며, 아밀로펙틴을 구성하는 포도당의 평균 사슬길이는 약간씩 증가하고 있었다. X선 회절도상에서는 4품종 모두 전형적인 A type이었으며, glucoamylase에 의한 가수분해도는 찰품종의 경우에는 거대배아미로 유기되면서 가수분해도가 낮아지는데 비해서 메품종의 경우에는 반대의 결과를 얻었다. 호화종료온도는 거대배아미 품종으로 유기됨에 따라 낮아지고 있었고, 호화 엔탈피는 거대배아미 품종으로 유기됨에 따라 증가하고 있었다.

Starches from 4 rice cultivars, including Whachungchalbyeo and Whachungbyeo, together with two correspondiug conversion mutants with giant embryonic phenotype, Whachungchal-giant embryonic rice and Whachung-giant embryonic rice, were isolated to compare its physico-chemical properties. The amylose content and the length of glucose chains in the fractionated amylose was reduced in response to conversion of the rice cultivars for developing giant embryonic phenotype. For the fine structure of amylopectin, conversion to giant embyonic rice cultivars accompanied with slight increase in the length of B chain plus decrease in the amount of A chain, and also a slight increase in average glucose chains length of amylopectin fractions could be observed. X-ray diffractogram revealed 4 cultivars to be the representative A types. We could also obtain the results that the hydrolysis rate by glucoamylase in the waxy rice cultivars decreased in response to conversion to the giant embryonic rice cultivars, while the rate increased in the non-waxy rice cultivas. Termination temperature for gelatinization was observed to decrease in response to conversion into the giant embryonic rice cultivars, however, the enthalpy for gelatinization was found to increase with the same conversion process.

키워드

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