한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제11권2호
  • /
  • Pages.148-154
  • /
  • 2003
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

흰쥐의 항산화 활성에 미치는 홍삼, 삼백초, 복분자 추출물의 상승효과

Synergistic Effects of extracts from Korean Red ginseng, Saururus chinensis(Lour.) Baill. and Rubus coreanus Miq. on Antioxidative Activities in Rats

  • 최면 (강원대학교 축산가공학과) ;
  • 신건재 (강원대학교 바이오산업공학부) ;
  • 최근표 (강원도립대학 식품생명과학과) ;
  • 도재호 (한국인삼연초연구원) ;
  • 김종대 (강원대학교 바이오산업공학부)
  • Choe, Myeon (Department of Animal Products Science, Korea Nutrition Institute, Kangwon National University) ;
  • Shin, Geon-Jae (School of Biotechnology and Bioengineering, Korean Nutrition Institute, Kangwon National University) ;
  • Choi, Geun-Pyo (Dept. of Food & Life Science, Gangwon Provincial University) ;
  • Do, Jae-Ho (Korea Ginseng Crop.) ;
  • Kim, Jong-Dai (School of Biotechnology and Bioengineering, Korean Nutrition Institute, Kangwon National University)
  • 발행 : 2003.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

홍삼(panax ginseng C.A. Meyer) 추출물 단독의 항산화 활성과 홍삼추출물에 삼백초(Saururus chinensis(Lour.) Baill.)와 복분자(Rubus coreanus Miq.) 추출물을 농도별로 보충 급여 하였을 때 나타나는 상승효과를 알아보기 위하여 Sprague Dawley rat에 이들 추출물을 8주간 섭취시킨 후 희생시켜 간장 cytosol중의 superoxide dismutase, catalase, glutathione peroxidase, GST 등의 항상화 효소계 활성을 측정하였다. 그 결과, 홍삼급여군의 superoxide dismutase, catalase, glutathione peroxidase의 효소활성이 대조군에 비해 농도 의존적으로 높았다. 특히 홍삼 단독보다는 삼백초와 복분자를 보충급여시 상승효과가 높았으며 급여기간이 길수록 그 효과가 높았다.

This study was designed to investigate the effect of Korean Red ginseng(Panax ginseng C.A. Meyer) extracts on antioxidative activities in Sprague-Dawley rats. This study also evaluated the synergistic effect of Korean red ginseng(RG) extracts with Saururus chinensis(Lour.) Baill(SC). and Rubus coreanus Miq.(RC) extracts. Experimental groups were divided into supplementation type(RG extracts, RG with SC and RC extracts) and amounts of extracts. Rats were received drinking water with or without RG, RC and SC extracts for eight weeks. In the antioxidant enzyme activities of liver cytosol, superoxide dismutase, catalase and glutathione peroxidase activities were significantly increased in RG groups and RG with SC and RC groups compared to control group. The antioxidative activities were increased in proportion to supplementation period and amounts of extracts. These results suggest that RG, RC and SC extracts have an beneficial effect to enhance the cellular antioxidant activities in rats.

키워드

참고문헌

  1. Abei H (1984) Catalase in vitro. Methods in Enzymology 105: 121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Aniya Y and Naito A (1993) Oxidative stress-induced activation of microsomal glutatbione-Sr-transferase in isolated rat liver. Biochem. Pharm. 45:37-43 https://doi.org/10.1016/0006-2952(93)90374-6
  3. Habig WH, Pabst MJ, Jakoby WB (1974) G1utathione-Stransferase. J. BioI. Chern. 249:7139
  4. Jones GL, Master CJ (1975) On the nature and characteristics of the multiple forms of catalase in mouse liver. Arch. Biochem. Biophys. 169:7-12 https://doi.org/10.1016/0003-9861(75)90311-2
  5. Kang YH, Park YK, Ha TY, Moon KD (1996) Effects of Pine Needle Extracts on Enzyme Activities of Serum and Liver , and Liver Morphology in Rats Fed High Fat Diet. J. Korean Soc. Food Nutr., 25(3):374-378
  6. Kim JS, Song SH, Kim KW (1995) The antioxidative mechanism of bismuth nitrate. Korean Soc. Gerontology, 5(2):101-110
  7. Kwon MJ, Joun YS, Song YO (1994) The Degree of Lipid Oxidation of Rat Liver Fed Peroxidized Lipid and Its Effects on Anti - Oxidative System. J. Korean Soc. Food Nutr., 23(6):899-907
  8. Lee SJ, Kim MJ, Yoon YH (1995) Effects of Korean green tea, oolong tea and black tea beverage on the removal of cadmium and antioxidative detoxification in cadmium administered rats. Proceedings of 3rd international symposium on green tea, Republic of Korea, pp 21-38
  9. Leopold F, Wolfgang AG (1984) Assays of Glutathione peroxidase. Methods in Enzymology 105: 114-121 https://doi.org/10.1016/S0076-6879(84)05015-1
  10. Levander OA (1983) Platelet glutathione peroxidase activity as an index of selenium ststus in rats. J. Nutr, 113:55-59 https://doi.org/10.1093/jn/113.1.55
  11. Lowry OH, Roserbrough NJ, Fiatt AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J. BioI. Chem. 193:265-275
  12. McCord JM and Fridovich I (1969) Superoxide dismutase: An enzymic function for erythrocupreinfl-lemocuprein). J. Biol, Chem. 244(22):6049-6055
  13. Park JW. Chung MH, Kim MS, Kim JS (1996) Oxidative stress and antioxidant enzyme activities in Senescence accelerated Mouse. Korean Soc. Gerontology 6(1):1-8
  14. Rhee SJ, Jang SY, Yang JA (1996) Effect of Dietary Vitamin E Levels on Lipid Peroxidation and Enzyme Activities of Antioxidative System in Brain of Cadmium Administered Rats. J. Korean Soc. Food Nutr., 25(4):575-580
  15. Smith GH, Ohl VS, Litwak G (1977) Ligandin, the glutathione- S-transferase and chemically induced hepatocarcinogenesis. Cancer Research 37:8-14