Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
권호 표지

Pretective Effect of Purple Sweet Potato (Ipomoea batatas) on Hepatotoxicity Rats Induced by Carbon Tetrachlolide

자색고구마가 사염화탄소 투여에 의한 흰쥐의 간손상 보호에 미치는 영향

  • Kim, Hyeon-A (Department of Food & Nutrition, Mokpo National University) ;
  • Bang, Mi-Ae (Department of Food & Nutrition, Mokpo National University) ;
  • Oh, Yong-Bee (Mokpo Experiment Sta. National Honam Agr. Experiment Sta.) ;
  • Jeong, Byeong-Choon (Mokpo Experiment Sta. National Honam Agr. Experiment Sta.) ;
  • Moon, Youn-Ho (Mokpo Experiment Sta. National Honam Agr. Experiment Sta.) ;
  • Jeong, Woo-Jin (Mokpo Experiment Sta. National Honam Agr. Experiment Sta.) ;
  • Cho, Young-Ja (Department of Food & Nutrition, Mokpo National University)
  • 김현아 (목포대학교 식품영양) ;
  • 방미애 (목포대학교 식품영양) ;
  • 오용비 (호남작물시험장 목포시험장) ;
  • 정병춘 (호남작물시험장 목포시험장) ;
  • 문윤호 (호남작물시험장 목포시험장) ;
  • 정우진 (호남작물시험장 목포시험장) ;
  • 조영자 (목포대학교 식품영양)
  • Published : 2003.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the effects of dietary purple sweet potato(Ipomoea batatas) powder on serum lipid levels and antioxidative enzymes in normal and pretective effect on hepatotoxicity rats induced by carbon tetrachlolide. Four groups of rats (3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(C), induced hepatotoxicity rats fed control diet(EC), normal rats fed purple sweet potato diet(P), and induced hepatotoxicity rats fed purple potato sweet diet(EP). Rats were induced by single injection of 50% carbon tetrachlolide(0.1 mL/100 g B.W., i.p.). The rats were fed ad libitum each of the experimental diet for 5 weeks. After 5 weeks the rats were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver homogenates. But serum concentrations of lipid was not significant in all groups. Serum alanine aminotransferase(ALT/GPT) and aspartate aminotransferase(AST/GOT) of the EC and EP groups were heigher than the C and P groups. The hepatic glucose 6-phosphatase(G6Pase) activity of the group fed purple potato diet(P) was lower than the other groups(p<0.05). However, The glutathione peroxidase(GPx) activities was not statistically different between the groups. Renal glutathione S-transferase(GST) activity of the EC and EP groups were lower than the C and P groups(p<0.05). In conclusion, these results suggest that purple sweet potato is believed to be possible protective effect on hepatotoxicity rats induced by carbon tetrachlolide.

Keywords

References

  1. Jackman RL, Yada RA, Jung MA, Spers RA. Anticyanin as food colorants. J Food Biochem 11: 204-247,1987
  2. Jackman RL, Yada RA, Jung MA. Separation and chemical properties of anthocyanin used for their qualitative and quantitative analysis. J Food Biochem 11:279-308, 1987 https://doi.org/10.1111/j.1745-4514.1987.tb00128.x
  3. Mazza G, Miniati E. Anthocyanins in fruits, vegetables and grains. CRC Press Inc Boca Raton Florida, p29-40,1993
  4. Francis FJ. Future trends, In Developemends in Food Color-2, Walfood J (ed) Applied Sciences Publishers, New York: p 233-247, 1984
  5. Francis FJ. Food colorants Amthocyanins. Crit Res Food SciNutr 28:273-314,1989 https://doi.org/10.1080/10408398909527503
  6. Imbert MP, Seaforth CE, William DB. The anthocyanin pigments of the sweet potato Ipomoea batatas. Proc Am Hortic Sci 288: 481-485, 1966
  7. Shi Z, Bassa LA, Gabriel SL,Fancis FJ. Anthocyanin pigments of sweet potato-Ipomoea batatas. J Food Sci 57: 755-757, 1992 https://doi.org/10.1111/j.1365-2621.1992.tb08088.x
  8. Kim SJ, Rhim JW, Lee LS, Lee JS. Extraction and characteristics of purple sweet potato pigments(in korean) Korean J Food Sci Technol 28: 345-351, 1996
  9. Lee LS ,Rhim JW, Kim SJ,Chung Be. Study on the stability of anthocyanin pigment extracted from purple sweet potato(in Korean). Korean J Food Sci Technol. 28:356-359, 1996
  10. Lee LS, Rhim JW, Kim SJ, Chung Be. Growth characteristics and changes of pigment content of purple sweet potato during growth (in Korean). Korean J Food Sci Technol 28: 1180-1183, 1996
  11. Kim SJ, Rhim JW. Characteristics of pigment extracted from purple sweet potato by nanofiltration(in Korean). Korean J Food Sci Technol 29: 492-496, 1997
  12. Lee LS, Rhim JW. Thermal kinetic of color changes of purple sweet potato anthocyanin pigment(in Korean). Korean J Food Sci Technol 29: 479-501, 1997
  13. Recknaged Ro. Carbon tetrachloride hepatotoxicity. phamacol Rev 19:145-208, 1967
  14. Recknaged RO, Glende EA. Carbon tetrachloride hepatotoxicity: an example of lethal cleavage. CRC Crit Rev ToxicoI 2:263-297, 1973 https://doi.org/10.3109/10408447309082019
  15. Villarruel MC, Diaz Gomez MI, Castro JA. The nature of the in vitro irreversible binding of carbon tetrachloride to microsomal lipids. Toxicol Appl Phamacol 33:106-114,1975 https://doi.org/10.1016/0041-008X(75)90249-5
  16. Glende EA, Pushpendam CK. Activation of phospholipase A2 by carbon tetrachloride in isolated rat hepartocytes. Biochem Phamacol 35:3301-3307, 1986 https://doi.org/10.1016/0006-2952(86)90427-2
  17. Long RM, Moore L. Inhibition of liver endoplasmic reticulum calcium pump by CCl4 and release of sequestered calcium pool. Biochem Phamacol 35:4131-4137,1986 https://doi.org/10.1016/0006-2952(86)90687-8
  18. Plaa GL, Hewitt WR. Quantative evaluation of indicies of hepatotoxicity. In Toxicology of the Liver. Zakim D, Boyer TD, eds Raven Press, New York. pp.l03-120, 1982
  19. Recknagel RO. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci 33: 401-408,1983
  20. Bucolo G, Gavid H. Quantitative determination of serum triglyceride by use of enzymes. Clin Chern 19:476-482,1973
  21. Friedwald WT, Levy RL, Fedreicson DS, Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without was of the preparative ultracentrifuge. Clin Chern 18: 499-506, 1972
  22. Baginski ES, Foa PP, Zak B. Glucose 6-phosphatase In Methods of Enzymematic Analysis Vol. 2. Academic Press, New York, pp876-880, 1983
  23. Buege JA, Aust SD, Sidney F, Lester P. Methods in Enzymology Vol. 52, Academic Press, New York, pp302-310, 1978 https://doi.org/10.1016/S0076-6879(78)52032-6
  24. Habig WH, Pabst MJ, Jakoby WB. Glutathione Stransferase. J.Biol. Chem. 249:7130-7139, 1974
  25. Tappel AL. Glutathione peroxidase and hydroperoxides, In Methods in enzymology (Fleisher S & Packer Led) 52:506-513,1978 https://doi.org/10.1016/S0076-6879(78)52055-7
  26. Lowry OH, Rosebrough NJ, Farr AL, Randall RT. Protein measurement with the folin phenol reagent. J Bioi Chem, 193:265-275, 1951
  27. Bang MA, Cho YJ, Kim HA. Effect of Indongcho (L.japonica Thumb)on glucose and lipid metabolism and antioxidative enzymes system in streptozotocin-induced diabetic rats. 17(4): 377-386, 2002
  28. Hayes. Principles and medthods of toxicology. Gabriel LP, William RH(eds), Raben Press, New York, p. 407-445,1982
  29. Vos RM, Van BlademPJ. Glutathione 5-transferase in relation to their role in the biotransfermation of xenobiotics. Chern Bioi Interact, 75: 241-265, 1990 https://doi.org/10.1016/0009-2797(90)90069-Y
  30. Jones DP, Eklow L Thor H, Orrenius S. Metabolisn of hydrogen peroxide in isolated hepatocytes relative contributions of catalase and glutathione peroxidase jn decomposition of endogeneously generated H2O2. Arch Biochem Biophys, 210;505-516,1981 https://doi.org/10.1016/0003-9861(81)90215-0
  31. Ghosh R, Mukherjee B, Chatterjee M. A novel effect of selenium on streptozotocin-induced diabetic mice. Diabetes Res 25(4):165-71,1994
  32. Argaud, D., Zhang, Q., Pan, W., Maitra, S., Pilkis, S. J.,and Lange, A. J. Diabetes 45,1563-1571,1996 https://doi.org/10.2337/diabetes.45.11.1563
  33. Trinh, K. Y., O' Doherty, R. M., Anderson, P., Lange, A. J., and Newgard, C. B. Perturbation of Fuel Homeostasis Caused by Overexpression of the Glucose-6-phosphatase Catalytic Subunit in Liver of Normal RatsJ.BioI.Chem. 273,31615-31620,1998 https://doi.org/10.1074/jbc.273.47.31615
  34. Liu Z, Barrett EJ, Dalkin AC, Zwart AD, Chou JY Effect of acute diabetes on the rat hepatic glucose-6-phosphatase activity and its messenger RNA level Biochem Biophy Res Commun 30;205(1) 680-686, 1994
  35. Kedziora-Kornatowska K, Luciak M. Effect of aminoguanidine on lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney iochem Mol Biol Int Oct;46(3):577-83, 1998
  36. Celik S, Baydas G, Yilmaz O. Influence of vitamin E on the levels of fatty acids and MDA in some tissues of diabetic rats. Cell Biochem Funct. 20(1):67-71,2002 https://doi.org/10.1002/cbf.936
  37. Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I.Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta DiabetoI 37(4): 179-8, 2000 https://doi.org/10.1007/s005920070002
  38. Curtis MI, Gilfor D, Farber JL. Lipid peroxidation increased the molecular order of microsomal membranes. Arch Biochem Biophy, 235: 644-651, 1984 https://doi.org/10.1016/0003-9861(84)90239-X
  39. Takeda S, Funo S, Iizuka A, Kase Y, Arai I, Ohkura Y, Sudo K, Kiuchi M, Yoshida C, Maeda S Abarada n, Hosoya E. Phamacological studies on schzandra . fruits. III. Effects of wuweizisu C, a ligan component of schixanda fruits, on experimental liver injuries in rats. Folia Phamacol Japan, 85:194-208,1985
  40. Noll T, Groot H. The critical steady state hypoxcic conditions in carbon tetrachlolide induced lipid peroxidation in rat liver microsomes. Biochem Biophy Acta, 795: 356-362, 1984 https://doi.org/10.1016/0005-2760(84)90086-9
  41. Hase K, Ohsugi M, Xiong Q Basnet P, Kadota S, Namba T. Hepatoprotective effectof Hovenia dulcis 1HUNB on experimental liver injuries induced by carbon tetrachlolide or D-galactosamine/ lipopolysaccharide. BioI Pharm Bull, 20; 381-385, 1997 https://doi.org/10.1248/bpb.20.381