Hollow Fiber 검색법과 Xenographic Animal Assay를 이용한 생약재의 암세포 저해활성 비교

Comparative Activity of Medicinal Herbs Between Hollow Fiber Assay and Xenographic Animal Assay

  • 발행 : 2003.12.30

초록

We compared the antitumor activity between hollow fiber assay and xenographic animal assay on thirty herbal plants. It is evaluated that the antitumor activity of above 30% is regarded as 'positive response', and its of below 30% is regarded as 'negative response'. The two herbal plants extracts (Ulmus davidiana Hedyotis diffusa) among thirty herbal plants show to be positive in xenographic animal assay and they were also correctly identified as positive by the hollow fiber assay. The correlation of the hollow fiber assay data with xenographic animal assay would suggest that hollow fiber assay presents a potentially unique tool to develop the herbal medicine for cancer.

키워드

참고문헌

  1. Rubinstein, L.V., Shoemaker, R.H., Paull, K.D., Simon, R.M., Tosini, S., Skehan, P., Scudiero, D.A., Monks, A., and Boyd, M.R. (1990) Comparison of in vitro anticancer-drugscreening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines. J. Natl Cancer Inst. 82(13): 1113-1118 https://doi.org/10.1093/jnci/82.13.1113
  2. Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J.T., Bokesch, H., Kenney, S., and Boyd, M.R. (1990) New colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl Cancer Inst. 82(13): 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  3. Borenfreund, E. and Puerner, J.A. (1984) A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/Nr-90). Tissue Culture Meth. 9: 7-9
  4. Hollingshead, M.G., Alley, M.C., Camalier, R.F., Abbott, B.J., Mayo, J.G., Malspeis, L., and Grever, M.R. (1995) In vivo cultivation of tumor cells in hollow fibers. Life Sci. 57(2): 131-141 https://doi.org/10.1016/0024-3205(95)00254-4
  5. Freije, J.M., Lawrence, J.A., Hollingshead, M.G., De la Rosa, A., Narayanan, V., Grever, M., Sausville, E.A., Paull, K., and Steeg, P.S. (1997) Identification of compounds with preferential inhibitory activity against low-Nm23-expressing human breast carcinoma and melanoma cell lines. Nat Med. 3(4): 395-401 https://doi.org/10.1038/nm0497-395
  6. Hall, L.A., Krauthauser, C.M., Wexler, R.S., Hollingshead, M.G., Slee, A.M., and Kerr, J.S. (2000) The hollow fiber assay: continued c characterization with novel approaches. Anticancer Res. 20(2A): 903-911
  7. Casciari, J.J., Riordan, N.H., Schmidt, T.L., Meng, X.L., Jackson, J.A., and Riordan, H.D. (2001) Cytotoxicity of ascorbate, lipoic acid, and other antioxidants in hollow fibre in vitro tumours. Br. J. Cancer, 84(11): 1544-1550 https://doi.org/10.1054/bjoc.2001.1814
  8. Krauthauser, C.M., Hall, L.A., Wexler, R.S., Slee, A.M., Mitra, J., Enders, G.H., and Kerr, J.S. (2001) Regulation of gene expression and cell growth in vivo by tetracycline using the hollow fiber assay. Anticancer Res. 21(2A): 869-872
  9. Malone, C.C., Schiltz, P.M., Mackintosh, A.D., Beutel, L.D., Heinemann, F.S., and Dillman, R.O. (2001) Characterization of human tumor-infiltrating lymphocytes expanded in hollow-fiber bioreactors for immunotherapy of cancer. Cancer Biother Radiopharm. 16(5): 381-390 https://doi.org/10.1089/108497801753354285
  10. Jayaraman, M., Fox, B.M., Hollingshead, M., Kohlhagen, G., Pommier, Y., and Cushman, M. (2002) Synthesis of new dihydroindeno[l,2-c] isoquinoline and indenoisoquinolinium chloride topoisomerase I inhibitors having high in vivo anticancer activity in the hollow fiber animal model. 1. Med.Chem. 45(1): 242-249 https://doi.org/10.1021/jm000498f
  11. Monga, M. and Sausville, E.A. (2002) Developmental therapeutics program at the NCI: molecular target and drug discovery process. Leukemia. 16(4): 520-526 https://doi.org/10.1038/sj.leu.2402464
  12. Sadar, M.D., Akopian, V.A., and Beraldi, E. (2002) Characterization of a new in vivo hollow fiber model for the study of progression of prostate cancer to androgen independence. Mol. Cancer Ther. 1(8): 629-637
  13. Casciari, J.J., Hollingshead, M.G., Alley, M.C., Mayo, J.G., Malspeis, L., Miyauchi, S., Grever, M.R., and Weinstein, J.N.(1994) Growth and chemotherapeutic response of cells in a hollow-fiber in vitro solid tumor model. J. Natl Cancer Inst.86(24): 1846-1852 https://doi.org/10.1093/jnci/86.24.1846
  14. Maruo, K., Ueyama, Y., lnaba, M., Emura, R., Ohnishi, Y., Nakamura, O., Sato, O., and Nomura, T. (1990) Responsiveness of subcutaneous human glioma xenografts to various antitumor agents. Anticancer Res. 10(1): 209-212
  15. Fruebauf, J.P. and Bosanquet, A.G. (1993) In vitro determination of drug response: a discussion of clinical applications. Principle and Practice of Oncology 7(12): 1-16
  16. Berger, D.P., Fiebig, H.H., Winterhalter, B.R., Wallbrecher, E., and Henss, H. (1990) Preclinical phase Il study of ifosfamide in human tumour xenografts in vivo. Cancer Chemother Pharmacol. 26 Suppl: S7-11 https://doi.org/10.1007/BF00685408