Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effects of Chelidonine on L-DOPA-Induced Cytotoxicity in PC12 Cells

Chelidonine이 PC12 세포내의 L-DOPA-유도 세포독성에 미치는 영향

  • Kim, Yu-Mi (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Jae-Joon (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Jin, Chun-Mei (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Yang, Yoo-Jung (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Kang, Min-Hee (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
  • 김유미 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 이재준 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 김춘매 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 양유정 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 강민희 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 이명구 (충북대학교 약학대학, 생물건강산업개발연구센터)
  • Published : 2003.09.30
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Abstract

The effects of chelidonine, a benzophenanthridine isoquinoline alkaloid, on L-DOPA-induced cytotoxicity in PC12 cells were investigated. The treatment of PC12 cells with chelidonine $(1-4\;{\mu}M)$ decreased dopamine content in a dose-dependent manner (30.2% inhibition at $4\;{\mu}M)$. Chelidonine was not cytotoxic up to $4\;{\mu}M)$. However, chelidonine at concentrations higher than $5\;{\mu}M$ caused a cytotoxicity in PC12 cells. L-DOPA at concentrations higher than $50\;{\mu}M$ led to cell damage by oxidative stress in PC12 cells. Chelidonine at non-cytotoxic concentration ranges of $1-4{\mu}M$ aggravated L- DOPA $(20-50\;{\mu}M)$-induced cytotoxicity in PC12 cells. The L-DOPA-induced cytotocxicity was synergistically stimulated by chelidonine at concentrations grader than $5\;{\mu}M$. These data demonstrate that chelidonine exacerbates L-DOPA-induced cytotoxicity. Therefore, it is proposed that the long-term L-DOPA therapeutic patients with chelidonine may need to be checked for the adverse symptoms.

Keywords

References

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