Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effects of Liriodenine on Dopamine Biosynthesis in PC12 Cells

Liriodenine이 PC12 세포중의 Dopamine 생합성에 미치는 영향

  • Jin, Chun-Mei (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University) ;
  • Lee, Jae-Joon (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University) ;
  • Yin, Shou-Yu (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University) ;
  • Kim, Yu-Mi (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University) ;
  • Kim, Young-Kyoon (College of Forest Science, Kookmin University) ;
  • Rhu, Shi-Yong (Korea Research Institute of Chemical Technology) ;
  • Lee, Myung-Koo (College of Pharmacy, and Research Center Bioresource and Health, Chungbuk National University)
  • 김춘매 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 이재준 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 윤수옥 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 김유미 (충북대학교 약학대학, 생물건강산업개발연구센터) ;
  • 김영균 (국민대학교 임산공학과) ;
  • 유시용 (한국화학연구원) ;
  • 이명구 (충북대학교 약학대학, 생물건강산업개발연구센터)
  • Published : 2003.03.30
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Abstract

The effects of liriodenine, an aporphine isoquinoline alkaloid, on dopamine content in PCl2 cells were investigated. Treatment of PC12 cells with liriodenine decreased dopamine content in a dose-dependent manner (33.6% inhibition at $10\;{\mu}M$ for 12 h). The $IC_{50}$ in value of liriodenine was $8.4\;{\mu}M$. Dopamine content decreased at 3 h and reached a minimal level at 12 h after the exposure to liriodenine. Under these conditions, the activities of tyrosine hydroxylase and aromatic L-amino acid decarboxylase were also inhibited at $10\;{\mu}M$ of liriodenine by 10.1% and 20.2% relative to control, respectively. In addition, liriodenine inhibited the increase in dopamine content induced by L-DOPA Treatments $(50-100\;{\mu}M)$ in PC12 cells. These results suggest that liriodenine inhibited dopamine biosynthesis and L-DOPA-induced increase in dopamine content by reducing the activities of tyrosine hydroxylase and aromatic L- amino acid decarboxylase in PC12 cells.

Keywords

References

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