Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of Porcine Pancreatic Elastase (PPE) by Korean Mistletoe (Viscum album var.coloratum) Fractions

  • Lyu, Su-Yun (School of Pharmaceutical Sciences, Boots Science Building, University of Nottingham) ;
  • Moon, You-Sun (College of Natural Sciences, Seoul Womes University) ;
  • Kwon, Young-Ju (College of Natural Sciences, Seoul Womes University) ;
  • Joo, Hye-Jin (College of Natural Sciences, Seoul Womes University) ;
  • Park, Won-Bong (College of Natural Sciences, Seoul Womes University)
  • Published : 2003.12.01
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Abstract

The serine proteases such as human leukocyte elastase (HLE) and porcine pancreatic elastase (PPE) are classified in the chymotrypsin family, and possibly the most destructive enzymes having the ability to degrade virtually all of the connective components in the body. In the present study, the extracts of water and methanol of Korean mistletoe (Viscum album var. coloratum) inhibited significantly the PPE activity. The fractions eluated on Amberlite XAD-2 from methanol extract were further purified on the repeated $SiO_2$ column chromatography and the fractions A, B and C were eluated. The fractions A, B and C at 3 mg/ml inhibited significantly the PPE activity up to 66%, 95% and 85%, respectively. In conclusion, the fraction A assumed as lignans or phenylpropanes, and fraction B and C assumed as triterpenoids showed the PPE inhibitory effects on the PPE and that these compounds in mistletoe may be used for treatment of pathological processes such as age-dependent tissue loss or inflammation.

Keywords

References

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