IMMUNE NETWORK
- 제2권4호
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- Pages.195-201
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- 2002
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
Identification of Functionally Different Rat IgE in RBL-2H3 Exocytosis
- Kim, Jin-Sub (Department of Parasitology, Korea University) ;
- Cho, Sungae (Department of Microbiology, Yonsei University) ;
- Joo, Kyoung-Hwan (Department of Parasitology, Korea University) ;
- Lee, Joon-Sang (Department of Parasitology, Korea University) ;
- Conrad, Daniel H. (Department of Microbiology and Immunology, Virginia Commonwealth University) ;
- Cho, Sung-Weon (Department of Parasitology, Korea University)
- Published : 2002.12.31
Abstract
Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-2H3 has been well documented in the rat mucosal mast cell line. Methods: RBL-2H3 cells sensitized with each kind of rat IgE was activated by cross-linking FcRI with B5 (monoclonal anti-rat IgE mouse IgG antibodies). The RBL-2H3 exocytosis was measured by analyzing the