Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Prevalence of paratuberculosis of dairy cattle in Kangwon area

강원지역 젖소의 요네병 감염실태

  • 김두 (강원대학교 수의학과) ;
  • 전관준 (강원대학교 수의학과) ;
  • 김종택 (강원대학교 수의학과) ;
  • 신광순 (충남대학교 수의과대학) ;
  • 신명균 (강원도 가축위생시험소) ;
  • 장국현 (강원도 가축위생시험소) ;
  • 김정기 (강원도 가축위생시험소) ;
  • 김옥성 (강원도 가축위생시험소) ;
  • 정재영 (강원도 가축위생시험소)
  • Accepted : 2002.01.09
  • Published : 2002.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to conduct diagnosis of bovine paratuberculosis in Kangwon area. Blood samples were collected from 2,261 dairy cows of 162 herds, and the ELISA and immunoblotting using recombinant 34KDa protein of M. paratuberculosis were conducted. The feces collected from dairy cows were cultured on HEY medium with mycobactin-J and PCR was conducted with washing solution of medium 4 weeks after culture. The ELISA had sensitivity of 83.3% and specificity of 96.7%. And the immunoblotting had sensitivity of 83.3% and specificity of 100%. Of the 2,261 dairy cows, 371 cows(16.4%) were positive in ELISA and 75 cows(3.3%) were positive in immunoblotting. And of the 162 herds, 109 herds(67.3%) had an apparent paratuberculosis prevalence by ELISA and 40 herds(24.7%) by immunoblotting. The geographic distribution of herds with paratuberculosis was not uniform. Of the 241 feces samples including 110 feces from ELISA positive cow, 9 feces were positive in culture and PCR. PCR was able to detect the growth of M. paratuberculosis as early as 4 weeks of culture.

Keywords

References

  1. Johne HA, Frothingham L. Ein eigenthuemlicher fall von tuberkulose beim rind. Tiermed Path, 27: 438-454, 1895
  2. Twort FW, Ingram WD. A method for isolating and cultivating Mycobacterium enteritidis chronicae pseudotuber-culosae bovis johnei and some expriments onthe preparation of a diagnostic vaccine for pseudotuberculosae enteritis of bovis. Proc Soc Lond, 84: 517-543, 1912
  3. Chiodini RJ, Van Kruiningen HJ, Van Merkel RS. Ruminant paratuberculosis (Johne's Disease): The current status and future prospects. Cornell Vet, 74: 218-262, 1984
  4. Vary PH, Andersen PR, Green E, et al. Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's Disease. J Clin Microbiol, 28: 933-937, 1990
  5. Billman-Jacobe H, Carrigan M, Cockram F, et al. A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne's disease incattle. Aust Vet J, 69: 25-28, 1992
  6. Collins MT, Gabric DM, de Lisle GW. Identification of two group of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J Clin Microbiol, 28: 1591-1596, 1990
  7. Whipple DL, Callihan DR, Jarnagin JL. Cultivation of Mycobacterium paratuberculosis from bovine fecal specimens and a suggested standardized procedure. J Vet Diagn Invest, 3: 368-373, 1991
  8. Colston A, McConnell I, Bujdoso R. Cloning and expression in Escherichia coli of DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis,the causative agent of Johne's disease. Microbiology, 140: 3329-3336, 1994
  9. el-Zaatari FA, Naser SA, Engstrand L, et al. Identification and characterization of Mycobacterium paratuberculosis recombinant proteins expressed in E coli. Curr Microbiol, 29:177-184, 1994
  10. Yokomizo Y, Yugi H, Merkal RS. A method for avoiding false-positive reactions in an enzyme-linked immunosorvent assay(ELISA) for the diagnosis ofbovine paratuberculosis. Jpn J Vet Sci, 47: 111-119, 1985
  11. Milner AR, Lepper AW, Symonds WN, et al. Analysis by ELISA and western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei. Res Vet Sci, 42: 140-144, 1987
  12. Tsai SJ, Hutchinson LJ, Zarkower A. Comparison of a dot immunobinding assay, enzyme-linked immunosorbent assay and immunodiffusion for serodiagnosis of paratuberculosis. Can J Vet Res, 53: 405-410, 1989
  13. Cox JC, Drane DP, Jones SL, et al. Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne's disease in cattle. Aust Vet J, 68: 157-160, 1991
  14. 이방환, 임봉호, 하영시 등. 국내발생의 소 파라결핵병 예에 대한 임상병리학적 추적조사 보고. 대한수의사회지 19: 8-20, 1983
  15. 전윤성, 이방환, 김종배 등. 소 유래 Mycobactin 의존성 항산성균의 분리동정. 대한수의학회지, 24: 53-63, 1984
  16. 김종만, 안종삼, 우승룡 등. 면역학적인 방법에 의한 한우와 유우의 요네병 발생조사. 대한수의학회지, 34: 93-97, 1994
  17. Kim D, Park HW. Expression of the C-terminal of 34KDa protein of Mycobacterium paratuberculosis. Korean J Vet Res, 40: 86-93, 2000
  18. Voller A, Bidwell DE, Bartlett A. The enzyme-linked immunosorbent assay (ELISA). Diagnostic Laboratories, Alexandria, Va, 1983
  19. Collins MT, Sockett DC, Goodger WJ, et al. Herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in Wisconsin.JAVMA, 204: 636-641, 1994
  20. Collins MT. Diagnosis of paratuberculosis. Vet Clin North Am Food Anim Pract, 12:357-371, 1996
  21. De Kesel M, Gilot P, Coene M, et al. Composition and immunological properties of the protein fraction of A36, a major antigen complex of Mycobacteriumparatuberculosis. Scand J Immunol, 36: 201-212, 1992
  22. Gilot P, De Kesel M, Machtelinckx L, et al. Isolation and sequencing of the gene coding for an antigenic 34-kilodalton protein of Mycobacterium paratuberculosis.J Bacteriol, 175: 4930-4935, 1993
  23. Collins MT, Sockett DC, Ridge S, et al. Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease. J Clin Microbiol, 29: 272-276, 1991
  24. De Kesel M, Gilot P, Misonne MC, et al. Cloning and expression of portions of the 34-kilodalton protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease. J Clin Microbiol, 31: 947-954, 1993
  25. Thoen CO, Baum KH. Current knowledge on paratuberculosis. JAVMA, 192: 1609-1611, 1988
  26. Johnson-Ifearulundu Y, Kaneene JB. Distribution and environmental risk factors for paratuberculosis in dairy cattle herds in Michigan. Am J Vet Res, 60: 589-596, 1999
  27. 김태종, 김윤식, 김재천 등. 분자생물학과 면역학적 방법에 의한 소 요네병 진단의 연구. 대한수의학회지 37: 349-358, 1997