Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Phylogenic Comparison of Viral Nervous Necrosis (VNN) Viruses Occurring Seed Production Period

해산어 종묘 생산 시기에 발생하는 바이러스성 신경괴사증 (VNN) 원인바이러스의 유전학적 비교

  • Kim Suk Ryol (Department of Fish Pathology, Yosu National University) ;
  • Jung Sung Ju (Department of Fish Pathology, Yosu National University) ;
  • Kim Young Jin (Department of Fish Pathology, Yosu National University) ;
  • Kim Jin Do (National Fisheries Research & Development Institute) ;
  • Jung Tae Sung (Lab of Fish & Shellfish Disease, College of Veterinary, Gyeongsang National University) ;
  • Choi Tae Jin (Department of Microbiology, Pukyong National University) ;
  • Yoshimizu Mamoru (Division of Marine Bioscience, Hokkaido University) ;
  • Oh Myung Joo (Department of Fish Pathology, Yosu National University)
  • 김석렬 (여수대학교 수산생명의학과) ;
  • 정성주 (여수대학교 수산생명의학과) ;
  • 김영진 (여수대학교 수산생명의학과) ;
  • 김진도 (국립수산과학원 여수수산 종묘시험장) ;
  • 정태성 (경상대학교 수의학과) ;
  • 최태진 (부경대학교 미생물학과) ;
  • ;
  • 오명주 (여수대학교 수산생명의학과)
  • Published : 2002.05.01
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Abstract

This study was performed both to explore the host of nervous necrosis virus (NNV) between mariculturing fish species and to examine the phylogenic position of the NNV in Korea. NNV was confirmed on the basis of histopathological and molecular biological examination, then VNN infection was Preyed from either moribund or dead fishes including red drum, Sciaenops ocellatus; oblong rock fish, Sebastes oblongus and flounder, Paralichthys olivaceus. As a result of sequencing for a part of ms, virus from red drum was showed $98\%, $97\%, $86\% and $74\% homology with oblong rock fish, grouper, Japanese flounder and striped jack, respectively. On the other hand, NNV from oblong rock fish was demonstrated $96\%, $85\% and $72\% homology with grouper, Japanese flounder and striped jack, respectively. NNV from red drum and oblong rock fish was exhibited phylogenically distant from the representative NNV, SJNNV originated from striped jack. On the contrary, the viruses appeared to be similar species with Taiwan NNV isolated from culturing grouper.

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References

  1. Bovo, G., T. Nishizawa, C. Maltese, F. Borghesan, F. Mutinelli, F. Montesi and S. De Mas. 1999. Viral encephalopathy and re-tinopathy of farmed marine fish species in Italy. Virus Res., 63, 143-146 https://doi.org/10.1016/S0168-1702(99)00068-4
  2. Fukuda, Y., H.D. Nguyen, M. Furuhashi and T. Nakai. 1996. Mass mortality of cultured sevenband grouper, Epinephelus Septemfas-ciatus associated with viral nervous necrosis. Fish Pathol, 31, 165-170 https://doi.org/10.3147/jsfp.31.165
  3. Kim, J.D., S.R. Kim, S.J. Jung, Y.J. Kim, T.S. Jung, T.J. Choi, S.W. Park and M.J. Oh, 2001. Occurrence of viral nervous necrosis (VNN) in red drum (Sciaenops ocellatus) larvae. J. Fish Pathol. 14, 91-95
  4. Munday, B.L. and T. Nakai. 1997. Special topic review: nodaviruses as pathogens in larval and juvenile marine finlish. World J. Microbiol. Biotechnol., 13, 375-381 https://doi.org/10.1023/A:1018516014782
  5. Nishizawa, T., K. Mori, T. Nakai, I. Furusawa and K. Muroga. 1994. Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Dis. Aquat. Org., 18, 103-107 https://doi.org/10.3354/dao018103
  6. Nishizawa, T., M. Furuhashi, T. Nagai, T. Nakai and K. Muroga. 1997. Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene. Appl. Environ. Microbiol, 63, 1633-1636
  7. Pavoletti, E., M. Prearo, M. Ghittino and C. Ghittino. 1998. Cases of viral nervous necrosis (VNN) in shy drum, Umbrina cirrosa with description of clinical symptomatology and anatomo-histo-pathological features. Boll. Soc. Ital. Patol. Ittica., 10, 24-33
  8. Sohn, S.G., M.A. Park, M.J. Oh and S.K. Chun. 1998. A fish noda-virus isolated from cultured sevenband grouper, Eponephelus septemfasciatus. J. Fish Pathol., 11, 97-104
  9. Tanaka, S., H. Aoki and T. Nakai. 1998. Pathogenicity of the noda-virus detected from diseased sevenband grouper, Epiaephelus septemfasciatus. Fish Pathol., 33, 31-36 https://doi.org/10.3147/jsfp.33.31
  10. Thiery, R., C. Arnauld and C. Delsert. 1999. Two isolates of sea bass, Dicentracrchus labrax L., nervous necrosis virus with distinct genomes. J. Fish Dis., 22, 201-207 https://doi.org/10.1046/j.1365-2761.1999.00164.x
  11. Watanabe, K., T. Nishizawa and M. Yoshimizu. 2000. Selection of brood stock candidates of barfin flounder using an ELISA Sys-tem with recombinant protein of barfin flounder nervous necrosis virus. Dis. Aquat. Org., 41, 219-223 https://doi.org/10.3354/dao041219
  12. Zafran, I. Koesharyani, F. Johnny, K. Yuasa, T. Harada and K. Hatai. 2000. Viral nervous necrosis in humpback grouper, Cromileptes aftivelis larvae and juveniles in Indonesia. Fish Pathol., 35, 95-96 https://doi.org/10.3147/jsfp.35.95

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