Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Detection of the Recombinant MotX Protein Vibrio fluvialis in Escherichia coli with Immuno-Gold Labeling Method

Immuno Gold 표지법을 이용한 대장균내 Vibrio fluvialis MotX 단백질의 존재 부위 결정

  • LEE Jong Hee (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Park Jae Hyun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim Sun Hoi (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • An Sun Hee (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kong In Soo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2002.07.01
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Abstract

The rotation of the flagellar motor is powered by the electrochemical gradient of specific ions across the cytoplasmic membrane. Recently, the gents of the Na'-driven motor have been cloned from marine bacterium of Vibrio sp. and some of the motor proteins have been purified and characterized. Also, motx gene encoding a channel component of the sodium type flagellar motor was identified from Vibrio Huuiaiis (KTCC 2473). The amino acid sequence of MotX protein from V, Huvialis shared 90, 85, $85\%$ identity with V, cholerae, V. alginolyticus, V parahaemolyticus, respectively. We have studied the localization of the expressed MotX protein in Escherichia coli by immune-gold labeling of ultra-thin frozen section. Our observation of the expressed protein indicated that MotX protein could be existed as attachment to inner membrane in E. coli.

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References

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