Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Organic acid production and phosphate solubilization by Enterobacter intermedium 60-2G

Enterobacter intermedium 60- 2G의 유기산 생성과 불용성인의 가용화

  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, College of Agriculture, Chonnam National University) ;
  • Hwangbo, Hoon (Division of Applied Bioscience and Biotechnology, College of Agriculture, Chonnam National University) ;
  • Kim, Yong-Woong (Division of Applied Bioscience and Biotechnology, College of Agriculture, Chonnam National University) ;
  • Kim, Hyo-Jeong (Division of Applied Plant Science, Institute of Biotechnology, College of Agriculture, Chonnam National University) ;
  • Park, Keun-Hyung (Division of Applied Bioscience and Biotechnology, College of Agriculture, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Plant Science, Institute of Biotechnology, College of Agriculture, Chonnam National University) ;
  • Seong, Ki-Young (Division of Applied Plant Science, Institute of Biotechnology, College of Agriculture, Chonnam National University)
  • 김길용 (전남대학교 농과대학 응용생물공학부) ;
  • 황보훈 (전남대학교 농과대학 응용생물공학부) ;
  • 김용웅 (전남대학교 농과대학 응용생물공학부) ;
  • 김효정 (전남대학교 농과대학 응용식물학부) ;
  • 박근형 (전남대학교 농과대학 응용생물공학부) ;
  • 김영철 (전남대학교 농과대학 응용식물학부) ;
  • 성기영 (전남대학교 농과대학 응용식물학부)
  • Received : 2002.02.09
  • Accepted : 2001.12.06
  • Published : 2002.02.28
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Abstract

A phosphate solubilizing bacterium. strain 60-2G, possessing a strong ability to solubilize insoluble phosphate was isolated from the rhizosphere of grass. On the basis of GC-FAME profile, carbon utilization pattern, and the DNA sequence of a conserved partial 16S rRNA gene, the 60-2G was identified as Enterobacter intermedium. The analysis by HPLC revealed that the strain 60-2G produced mainly gluconic and 2-ketogluconic acids with small amounts of lactic acid in broth culture medium containing hydroxyapatite. During the incubation period of the strain 60-2G in broth culture, pH of the medium decreased upto 3.8 while the soluble phosphate concentration increased. The reversed correlation between pH and soluble phosphate concentration indicated that the solubility of P was due to the produced organic acids. The sequence homology of the deduced amino acids suggested that E. intermedium 60-2G synthesized PQQ which is essential for the oxidation of glucose by glucose dehydrogenase.

강한 인산 가용력을 가진 인산 용해 세균인 균주 60-2G를 잔디의 근권에서 분리하였다. GC-FAME구조와 탄소이용형태 및 16S rRNA의 부분 염기서열 분석을 통해 균주 60-2G는 Enterobacter intermedium으로 동정되었다. Hydroxyapatite를 첨가한 배지와 생장 시킨 균주 60-2G는 gluconic acid 와 2-ketogluconic acid 및 소량의 lactic acid를 생성하였다. 균주 60-2G의 생장 기간동안 배지의 pH는 3.8 까지 낮아지는 반면에 배지의 유효 인산 농도는 증가하였다. 배지의 낮은 pH와 유효인산농도의 증가는 역 상관관계이며, 이는 균주 60-2G가 생성하는 유기산에 의한 영향이다. E. intermedium 60-2G 균주는 유기산 생성에 관여하는 glucose dehydrogenase의 co-factor인 PQQ를 생성하였으며, pqq의 부분 염기서열 분석 결과 기존에 보고된 서열과 85% 이상의 상동성을 가지고 있었다.

Keywords

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