Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지

Spermatogenesis and Sperm Ultrastructure of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae)

대복, Gomphina veneriformis의 정자형성과정 및 정자 미세구조

  • Park, Chae-Kyu (Yeonam High School) ;
  • Park, Jung-Jun (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Yosu National University) ;
  • Lee, Jeong-Yong (Gangneung Hatchery, National Fisheries Research and Development Institute) ;
  • Lee, Jung-Sick (Department of Aqualife Medicine, College of Fisheries and Ocean Science, Yosu National University)
  • 박채규 (여남고등학교) ;
  • 박정준 (여수대학교 수산해양대학 수산생명의학과) ;
  • 이정용 (국립수산과학원 강릉수산종묘시험장) ;
  • 이정식 (여수대학교 수산해양대학 수산생명의학과)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Spermatogenesis and sperm ultrastructure are investigated by means of light and transmission electron microscopy in the equilateral venus, Gomphina veneriformis which is dominant bivalve in the east coast of Korea. In the active spermatogenic season, testis consists of numerous spermatogenic follicles which is contains germ cells in the different developmental stage. The spermatogonia attached to spermatogenic follicle wall and has a large nucleus with electron-dense nucleolus. The spermatocytes are characterized by appearance of synaptonemal complex and well-developed Golgi complex. Nucleus of spermatid consists of numerous heterogeneous granules with high electron density. Karyoplasmic condensation, acrosome and flagellum formations are observed during spermiogenesis. Testicular matured sperms of sperm bundle consists of head, midpiece and tail. The head is about $8.5{\mu}m$ long and comprises a long nucleus and a bullet-like acrosome ($8.5{\mu}m$ in length). Acrosomal rod of microfilaments is observed in the lumen between nucleus and acrosome. The midpiece has four mitochondria. And tail has the typical '9+2' microtubule system.

우리나라 동해안에 가장 많이 서식하는 조개류인 대복의 정소구조와 정자형성과정을 광학현미경과 투과전자현미경으로 조사한 결과는 다음과 같다. 대복의 정소는 소성결합조직으로 구성된 다수의 정자형성 소낭을 가진다. 동일한 정자형성 소낭 내에서는 여러 단계의 생식세포들이 관찰되었다. 정원세포들은 정자형성 소낭벽에 부착되어 있으며, 커다란 핵과 뚜렷한 인을 가진다. 정모세포에서는 연접사복합체와 골기체의 발달을 확인할 수 있었다. 정세포의 핵은 전자밀도가 높은 과립상의 염색질로 구성되며, 정자변태과정 동안에 핵의 응축 및 첨체와 편모의 형성을 관찰할 수 있었다. 정소 내에서 완숙 정자들은 다발을 형성하고 있으며, 두부, 중편, 미부로 구성되어 있었다. 두부의 길이는 약 $8.5{\mu}m$로, 첨체부와 핵 부위로 구분된다. 첨체는 길이 약 $1.1{\mu}m$의 총알형태였다. 두부와 첨체 사이에서는 미세섬유로 구성된 첨체기둥이 확인되었다. 중편에는 4개의 미토콘드리아를 가지며, 꼬리의 횡단면은 "9+2"의 구조를 나타냈다.

Keywords

References

  1. Bernard RTF, Hodgson AN: The fine structure of the sperm and spermatid differentiation in the brown mussel Perna perna. S Afr J Zool 20: 5-9, 1985 https://doi.org/10.1080/02541858.1985.11447904
  2. Chung EY, Kim YM, Lee SG: Ultrastructure study of germ cell development and reproductive cycle of the purplish Washington clam Saxidomus purpuratus. The Yellow Sea 5: 51-58, 1999
  3. Chung EY, Kim YM: Ultrastructure study of germ cell development and sexual maturation of the hard clam, Meretrix rusoria (Bivalvia: veneridae), on the west coast of Korea. J Med & Appl Malacol 10: 181-202, 2000
  4. Chung EY, Park GM, Kim BS: Ultrastructure of germ cell development and sexual maturation of the shortnecked clam, Ruditapes philippinarum (Bivalvia: Veneridae), on the west coast of Korea. The Yellow Sea 4: 17-29, 1998
  5. Dohnen MR: 1. Gametogenesis. In: Verdonk NH, Van Den Biggelaar JAM, Tompa AS, eds, The Mollusca, Vol. 3. Development, 1-48, Academic Press, New York, 1983
  6. Dorange G, Pennec M Le: Ultrastructural characteristics of spermatogenesis in Pecten maximus (Mollusca: Bivalvia). Invert Reprod Dev 15: 109-117, 1989 https://doi.org/10.1080/07924259.1989.9672031
  7. Franzén $\AA$: On spermiogenesis, morphology of the spermatozoon, and biology of fertilization among invertebrates. Zool Bidr Uppsala 31: 355-482, 1956
  8. Franzén $\AA$: Ultrastructural studies of spermatozoa in three bivalve species with notes on evolution of elongated sperm nucleus in primitive spermatozoa. Gamete Res 7: 199-214, 1983 https://doi.org/10.1002/mrd.1120070302
  9. Hodgson AN, Bernard RTF: Ultrastructure of the sperm and spermatogenesis of three species of mytilidae (Mollusca: Bivalvia). Gamete Res 15: 123-135, 1986 https://doi.org/10.1002/mrd.1120150204
  10. Jamieson BGM: Fish evolution and systematics: Evidence from spermatozoa. Cambridge University Press, New York, P. 319. 1991
  11. Kim JH: Spermatogenesis and comparative ultrastructure of spermatozoa in several species of Korean economic bivalves (13 Families, 34 Species). Thesis Ph D Pukyong Nat`l Univ P. 161, 2001
  12. Lee JY, Park YJ, Chang YJ: Gonadal development and reproductive cycle of Gomphina melanaegis (Bivalvia: Veneridae). J Korean Fish Soc 32(2): 198-203, 1999
  13. Morse MP, Zardus JD: Bivalvia. In: Harrison FW, Kohn AJ, eds, Microscopic anatomy of invertebrates. Vol 6A, pp. 7-118, Wiley-Liss Inc, New York, 1997
  14. Popham JD: Comparative spermatozoon morphology and bivalve phylogeny. Malacol Rev 12: 1-20, 1979
  15. Yasuzumi G: Electron microscope studies on spermatogenesis in various animal species. Int Rev Cytol 37:53-119, 1974 https://doi.org/10.1016/S0074-7696(08)61357-1
  16. O Foighil D: Fine structure of Lasaea subviridis and Mysella tumida sperm (Bivalvia: Galeommatacea). Zoomorphology 105: 125-132, 1985 https://doi.org/10.1007/BF00312147