Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Therapeutic Effects of Safflower (Carthamus tinctorius L.) Seed Powder on Osteoporosis

홍화 (Carthamus tinctorius L.)씨 분말의 골다공증 치료효과

  • Bae, Chun-Sik (College of Veterinary Medicine, Chonnam National University) ;
  • Park, Chang-Hyun (Electron Microscope Laboratory, College of Medicine, Korea University) ;
  • Cho, Hyung-Jin (College of Veterinary Medicine, Chonnam National University) ;
  • Han, Hye-Jeong (College of Veterinary Medicine, Chonnam National University) ;
  • Kang, Seong-Soo (College of Veterinary Medicine, Chonnam National University) ;
  • Choi, Seok-Hwa (College of Veterinary Medicine, Chungbuk National University) ;
  • Uhm, Chang-Sub (Dept. of Anatomy, Institute of Human Genetics, College of Medicine, Korea University)
  • 배춘식 (전남대학교 수의과대학) ;
  • 박창현 (고려대학교 의과대학 전자현미경실) ;
  • 조형진 (전남대학교 수의과대학) ;
  • 한혜정 (전남대학교 수의과대학) ;
  • 강성수 (전남대학교 수의과대학) ;
  • 최석화 (충북대학교 수의과대학) ;
  • 엄창섭 (고려대학교 의과대학 해부학교실, 유전병연구소)
  • Published : 2002.09.01
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Abstract

The therapeutic effect of safflower seed powder on experimental osteoporosis in the rat induced by ovariectomy was evaluated. Thirty Sprague-Dawley rats were ovariectomized at the age of 12 weeks. Seven weeks postovariectomy, rats were divided into two groups: control and safflower seed powder treated group. Five animals from each group were sacrificed at the following time points: 1, 3, and 5 weeks. Scanning electron microscopic observation and morphometric analysis of the tibiae epiphysis showed that the administration of safflower seed powder significantly prevented reduction of cortical bone width and bone volume compared with the control group. In conclusion, safflower seed powder contains something that prevent bone loss due to estrogen deficiency, and was effective in preventing the osteoporotic decrease of bone mass.

홍화씨는 한방 및 민간에서 뼈에 우수한 작용이 있는 것으로 알려져 오랫동안 복용되어 왔다. 최근 고령화 사회를 맞이하면서 골다공증 문제는 사회적인 문제로 인식되고 있으며 저자들이 기존에 실시한 실험결과에 의하면 홍화씨 분말은 난소를 적출한 rat에서 골다공증의 예방효과를 나타내었다. 그러나 아직까지 골다공증이 유발된 rat에서 홍화씨 분말이 미치는 효과에 관한 실험적 자료가 제시된 것은 별로 없다. 이에 저자들은 홍화씨 분말이 골다공증의 치료에 미치는 효과를 알아보기 위하여 본 실험을 실시하였다. 실험동물은 체중 230 g의 12주령의 Sprague-Dawley Rats를 사용하였으며, 양쪽 난소제거 후 7주부터 홍화씨 분말을 매일 0.3 g씩 복용시키면서 1, 3 및 5주 후에 경골을 채취하여 관찰하였다. 채취된 조직은 통상적인 주사전자현미경 시료제작법으로 고겅과정을 거친 후 10% 질산으로 12시간 탈회하여 뼈의 단면을 노출시키고 탈수, 건조 및 금도금 과정을 거쳐 주사전자현미경(Hitachi, S-450)으로 관찰하여 촬영하였다. 관찰결과 대조군은 골수강에서 피질골까지의 두께의 감소와 골수강의 확장이 심하였으나, 홍화씨 분말을 투여한 실험군에서는 1주에서 5주까지 거의 같은 소견을 나타내었다. 이상의 결과를 종합해보면 홍화씨 분말은 여성호르몬 결핍으로 인한 골다공증의 치료에 효과가 있는 것으로 사료된다.

Keywords

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