Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Moxibustion to Zusanli(ST36) on Alteration of Natural Killer cell Activity in rat spleen

족삼리혈 뜸자극이 흰쥐 비장 자연살해세포 활성에 미치는 영향

  • Choi Gi Soon (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Oh Sang Deog (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Han Jae Bok (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Lee Gi Seog (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Park Joan Ha (Department of East-West Medicine, Graduate School, Kyunghee University) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Jung Sung Ki (Department of Internal Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Ahn Hyun Jong (Department of Microbiology, College of Medicine, Kyunghee University) ;
  • Cho Young Wuk (Department of Physiology, College of Medicine, Kyunghee University) ;
  • Min Byung Il (Department of East-West Medicine, Graduate School, Kyunghee University)
  • 최기순 (경희대학교 대학원 동서의학과) ;
  • 오상덕 (경희대학교 대학원 동서의학과) ;
  • 한재복 (경희대학교 대학원 동서의학과) ;
  • 이기석 (경희대학교 대학원 동서의학과) ;
  • 박준하 (경희대학교 대학원 동서의학과) ;
  • 배현수 (경희대학교 한의과대학 생리학교실) ;
  • 정승기 (경희대학교 한의과대학 내과학교실) ;
  • 안현종 (경희대학교 의과대학 미생물학교실) ;
  • 조영욱 (경희대학교 의과대학 생리학교실) ;
  • 민병일 (경희대학교 대학원 동서의학과)
  • Published : 2002.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Moxibustion is one of major healing technique in oriental medicine. It has been widely used in many diseases such as rheumatoid arthritis, Hashimoto disease, breech presentation, etc. However, till now, effects of moxibustion on NK cell activity and relations between sympathetic nerve system(SNS) and the immune alteration induced by moxibustion were not well studied. This study was designed to evaluate effects of moxibustion on NK cell activity and the intervention of SNS in the alteration of NK cell activity induced by moxibustion. Splenic NK cytotoxity was measured in a standard 4-h 51Cr release assay. We measured the NK cytotoxity at after moxibustion stimulation for 1,3,5, and 7 days, and also measured the NK cell cytotoxity after 3 and 7 days burn stimulation with similar temperature. IL-2, IL-4, INF-γ in serum were measured by rat IL-2, IL-4, INF-γ ELISA TEST KIT. To evaluate the effects of sympathectomy on alteration of NK cell cytotoxity, 6-hydroxydopamine(6-OHDA : 5Omg/kg) was used. We showed that NK cell activity of moxibustion stimulation group increased at the 3rd day, and declined at 7th day in comparison with that of contol group. In moxibustion stimulation group, NK cell activity of 3 day stimulation group was significantly higher than sham group. On the contrary, in burn stimulation group, NK cell activity was significantly higher than that of sham groups at 3rd, 7th days. Patterns between moxibustion and burns were different. INF- γ level of 3 days moxibustion stimulation group significantly higher than sham group. IL 2 level among groups were not different. IL-4 was not detected in serum with this method. Sympathectomy abolished the NK cell activity alteration induced by moxibustion. The results suggest that moxibustion induces the alteration of NK cell activity, along with INF-γ and SNS is related to these effects.

Keywords

References

  1. J of Tradit Chin Med v.13 Effect of acupuncture and point-injection treatment on immunologic function in rheumatoid arthritis. Liu X;Sun L;Xiao J;Yin S;Liu C;Li Q;Li H;Jin B.
  2. J Tradit Chin Med v.13 A study om the clinical effect and immunological mechanism in the treatment of Hashimoto's thyroiditis by moxibustion Hu G;Chen H;Hou Y;He J;Cheng Z;Wang R
  3. Am J Chin Med v.29 Treatment of breech presentation. Kanakura Y;Kometani K;Nagata T;Niwa K;Kamatsui H;Shinzato Y;Tokunaga Y https://doi.org/10.1142/S0192415X01000058
  4. Am J Chin Med v.17 no.3-4 Effects of moxibustion on cellular immunocompetence of gamma- irradiated mice. Hau DM;Wu JC;Chang YH;Hwang JT https://doi.org/10.1142/S0192415X89000243
  5. Am J Chin Med v.17 Suppression of the DTH reaction in mice by means of moxibustion at electro-permeable points. Tohya K;Mastrogiovanni F;Sugata R;Ohnishi M;Kuroiwa K;Toda S;Kimura M;Kawamata J https://doi.org/10.1142/S0192415X8900022X
  6. Zhen Ci Yan Jiu v.21 Studies on anti-inflammatory and immune effects of moxibustion. Tang Z;Song X;Li J;Hou Z;Xu S
  7. Zhen Ci Yan Jiu v.21 Regulation on ACTH, beta-EP and immune function by moxibustion on different acupoints. Zhai D;Din B;Liu,R;Hua,X;Chen H
  8. Zhen Ci Yan Jiu v.19 Regulation on beta-END in tumor-bearing mice by moxibustion on Guanyuan point. Zhai D;Chen H;Wang R;Hua X;Ding B;Jiang Y.
  9. Zhen Ci Yan Jiu v.17 Experimental study on the influence of acupuncture and moxibustion on interleukin-2 in patients with rheumatoid arthritis. Xiao J;Liu X;Sun L;Ying S;Zhang Z;Li Q;Li H;Jin B;Wang S.
  10. Science v.214 Natural killer cell :their roles in defence against disease. Herberman RB;Ortaldo JR https://doi.org/10.1126/science.7025208
  11. Brain Res Rev v.23 The role adrenocorticoid as modulators of immune function in health and disease : neural, endocrine and immune interactions. Bruce SM;Christine AB;Kenneth WB;Karren B;William HC;Firdaus SD;Ronald HG;Richard PK;Andrew HM;Robert LS;Jay MW https://doi.org/10.1016/S0165-0173(96)00012-4
  12. Chem. Immun. v.47 Rgeting and activation of cytotoxic lymphocytes. Segal DM;Snider DP https://doi.org/10.1159/000318919
  13. J Leukoc Biol v.58 Cellular and molecular mechanism of INF-gamma production induced by IL-2 and IL-12 in a human NK cell line. Ye J;Ortaldo JR;Conlon K;Winkler-Pickett R;Young HA
  14. Science v.223 Opioid peptide mediate the suppressive effect of stress on natural killer cell cytotoxity. Shavit Y;Lewis JW;Terman GW https://doi.org/10.1126/science.6691146
  15. Nature v.138 A syndrome produced by diverse nocuous agents Seyle HA
  16. Burns v.27 Susceptibility of thermally injured mice to cytomegalovirus infection. Hiroyuki K;Makiko K;David NH;Richard BP;Fujio S https://doi.org/10.1016/S0305-4179(01)00028-6
  17. Immunol Today v.14 A Th1?Th2 switch is a critical step in the etiology of HIV infection. Clerici M;Shearer GM https://doi.org/10.1016/0167-5699(93)90208-3
  18. Trends Microbiol v.2 The role of Th1 and Th2 subsets in human infectious diseases. Del Prete G;Romagnani S https://doi.org/10.1016/0966-842X(94)90336-0
  19. J Pharmacol Exp Ther v.255 Sympathetic nervous systerm mediates central corticotropin-releasing factor induced suppression of natural killer cytotoxicity. Irwin M.;Hauger R. L.;Jones L.;Provencio M.;Britton K. T.
  20. Endocrinology v.131 Central corticotropin- releasing hormone activates sympathetic nervous system and reduces immune function : increased responsivity of aged rats. Irwin M;Hauger RL;Brown M https://doi.org/10.1210/en.131.3.1047
  21. FASEB J v.5 Neuropeptide Y and natural killer cell activity : finding in depression and Alzheimer caregiver stress. Irwin M;Brown M;Patterson T;Hauger R;Mascovich A;Grant I
  22. Am J Phsiol v.265 Central interferon- alpha inhibits natural killer cytotoxity through sympathetic innervation. Take S;Mori T;Katafuchi T;Hori T
  23. Neurosci Lett v.63 Theodorsson-Norheim E, Hamberger B : Frequency- and reserpine- dependant chemical coding sympathetic transmission : differential release of noradrenaline and neuropeptide Y from pig spleen. Lundberg JM;Rudehill A;Sollevi A https://doi.org/10.1016/0304-3940(86)90020-0
  24. Brain Behav Immun v.5 Neuropeptide Y innervation of the rat spleen: another potential immunomodulatory neuropeptide. Romano TA;Felten SY;Felten DL;Olschowska JA https://doi.org/10.1016/0889-1591(91)90011-X
  25. Annu Rev Immunol v.7 Th1 and Th2 cells: different patterns of lymphokine secretion lead to different functional properties. Mosmann TR;Coffman RL https://doi.org/10.1146/annurev.iy.07.040189.001045
  26. Int Arch Allergy Immunol v.113 An update on human Th1 and Th2 cells. Romagnani S;Parronchi P;DElios MM(et. al.) https://doi.org/10.1159/000237532
  27. J Immunol v.136 Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. Mosmann TR, Cherwinski H;Bond MW;Giedlin MA;Coffman RL.
  28. Immunol Today v.12 The role of T-cell subsets and cytokines in the regulation of infection. Scott P;Kaufmann SHE https://doi.org/10.1016/0167-5699(91)90063-Y
  29. Brain Behav Immun v.11 Neurotransmitter signaling of cells of the immune system : important progress major gaps. Felten DL https://doi.org/10.1006/brbi.1997.0490
  30. Immunol. Rev. v.100 Nonadrenergic sympathetic neural interaction with immune system : structure and function. Felten D;Felten S;Bellinger DL;Carlson SL;Ackerman KD;Madden K;Olschowski JA;Livnat S. https://doi.org/10.1111/j.1600-065X.1987.tb00534.x
  31. J Neuroimmunol. v.49 Sympathetic nerve system modulation of immune system. Ⅱ: Induction of lymphocyte proliferation and migration in vivo by chemical sympathectomy. Madden K;Felten S;Felten D;Hardy C;Livnat S https://doi.org/10.1016/0165-5728(94)90182-1
  32. J Neuroimmunol v.114 Chemical sympathectomy increases the innate immune response and decreases the specific immune response in the spleen to infection with Listeria monocytogenes. Rice PA;Boehm GW;Moynihan JA;Bellinger DL;Stevens SY https://doi.org/10.1016/S0165-5728(00)00421-5
  33. J Neuroimmunol v.103 no.2 Alterations in T lymphocyte activity following chemical sympathectomy in young and old Fischer 344 rats. Madden KS;Stevens SY;Felten DL;Bellinger DL. https://doi.org/10.1016/S0165-5728(99)00243-X
  34. J Immunol v.155 Alterations in cytokine and antibody production following chemical sympathectomy in two strains of mice. Kruszewska B;Felten SY;Moynihan JA
  35. Immunochemistry v.8 Engvall E. Perlman, P : Enzyme-linked immunosorbent assay(ELISA): Quantitative assay of immunoglobulin G. Engvall E.;Perlman, P https://doi.org/10.1016/0019-2791(71)90454-X
  36. Nature v.291 Interleukin-2 augment natural killer cell activity. Henney CS;Kuribayashi K;Kern DE;Gillis S https://doi.org/10.1038/291335a0