Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Subunit Assembly of Laminin Variants in Cultured BAEC

BAEC세포에서의 Laminin 이형체 Subunit의 회합에 관한 연구

  • Jeon Hoon (Department of Oriental Pharmacy. Woosuk University) ;
  • Leem Kang hyun (Department of Oriental Pharmacy. Woosuk University)
  • 전훈 (우석대학교 약학대학 한약학과) ;
  • 임강현 (우석대학교 약학대학 한약학과)
  • Published : 2002.08.01
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Abstract

Bovine aortic endothelial cells(BAEC) produce two variant forms of laminin with a subunit composition of AB1B2 and A'B1B2. Analyses of the intracellular assembly of these subunits revealed that the B1B2 dimer formed first, and that A or A' joined to form the AB1B2 or A'B1B2 trimer. Angiostatic steroids shifted the relative size of the A and A' monomer pool in BAEC, and competition between the A and A' subunits in joining the B1B2 dimer produced AB1B2 and A'B1B2 in different ratios. This result suggests that subunit replacement is the general mechanism for producing laminin variants by various cells for tissue morphogenesis. When laminin subunits in BAEC were cross-linked with dithio-bis-succinimidylpropionate(DSP) and immunoprecipitated with anti-Iaminin antiserum, monomeric A,A',B1 and B2 monomers and the B1B2 dimer migrated as extremely large molecules in sodium dodecyl sulfate gel electrophoresis under nonreducing conditions. When the crosslinking disulfide bonds were cleaved under reducing conditions, they migrated as the usual subunits. This result suggests that molecular chaperones were involved in the process of the assembly and replacement of laminin subunits.

Keywords

References

  1. J.Cell Biol. v.97 Capillary endothelial cell cultures;phenotypic modulation by matrix components. Madri,J.A.;Williams,S.K.
  2. Lab.Invest. v.55 Endothelial cell proliferation during angiogenesis. Form,D.M.;Pratt,B.M.;Madri,J.A.
  3. J.Mol.Biochem. v.150 Shapes, domain organization and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. Engel,J.;Odermatt,E.;Engel,A.;Madri,L.A.;Furthmayr,H.;Rohde,H.;Timpl,R.
  4. J.Biol.Chem. v.263 Human laminin B2 chain. Pikkarainen,T.;Kallunki,T.;Tryggvason, K.
  5. J.Biol.Chem. v.263 Laminin, a multidomain protein. Sasaki,M.;Kleinman,H.K.;Huber,H.;Deutzmann,R.;Yamada,Y.
  6. Proc.Natl.Acad.Sci.U.S.A. v.87 Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Ehrig,K.;Leivo,I.;Argraves,W.S.;Rouslahti,E.;Engvall, E.
  7. Nature v.338 A laminin- like adhesive protein concentrated in the synaptic cleft of the neuromuscular junction. Hunter,D.D.;Shah,Y.;Merlie,J.P.;Sanes,J.R.
  8. J.Cell Biol. v.114 Kalinin; An epithelium specific basement membrane adhesion molecule that is a component of anchoring filaments. Rousselle,P.;Lunstrum,G.P.;Keene,D.R.;Burgeson, R.E.
  9. J.Cell Biol. v.119 The dermal-epidermal junction of human skin contains a novel laminin variant. Marinkovich,M.P.;Lunstrum,G.P.;Keene,D.R.;Burgeson, R.E.
  10. FASEB J., v.4 Structure and function of laminin; anatomy of a multidomain glycoprotein. Beck,K.;Hunter,I.;Engel,J.
  11. J.Biol.Chem. v.265 Production of two variant laminin forms by endothelial cells and shift of their relative levels by angiostatic steroids. Tokida,Y.;Aratani,Y.;Morita,A.;Kitagawa,Y.
  12. Proc. Natl, Acad, Sci.U.S.A. v.73 Clonal growth of bovine aortic endothelial cells; Fibroblast growth factor as a survival agent. Gospodrowwicz,D.;Moran,J.;Braun,D.;Birdwell, C.
  13. J.Cell Biol. v.117 Involvement of the stress protein HSP 47 in procollagen processing in the endoplasmic reticulum. Nakai,A.;Satoh,M.;Hirayoshi,K.;Nagata,K.
  14. J.Biol.Chem. v.263 Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and production of unorthodox laminin complex. Aratani,Y.;Kitagawa,Y.
  15. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacterophage T4. Laemmli,U.K.
  16. Biochemistry v.26 In vivo cross-linking of protein disulfide isomerase to immunoglobulins. Roth,R.A.;Pierce,S.R.
  17. Biochem.J. v.229 Post-translational assembly and glycosylation of laminin subunits in parietal endoderm-like F9 cells. Morita,A.;Sugimoto,E.;Kitagawa,Y.