Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
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The Effects of ALA-PDT on Leukemia Cells and Hepatoma Cells

  • Chen, Ji-Yao (Department of Physics, Fudan University) ;
  • Ren, Qing-Guang (Department of Physics, Fudan University) ;
  • Wu, Su-Min (Department of Physics, Fudan University) ;
  • Wang, Pei-Nan (State Key Lab for Advance Photonic Materials and Devices, Fudan University)
  • Published : 2002.08.01
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Abstract

S-aminolevulinic acid (ALA) is a new kind drug used in photodynamic therapy. ALA-PDT have successfully used in superficial malignancies and some skin diseases. Here the effects of ALA-PDT were studied on leukemia cells and hepatoma cells to explore the application on different kind cancers. It was found from the fluorescence emission spectra, that after ALA incubation the sensitizer - protoporphyrin IX (PpIX) was endogenously produced in both leukemia and hepatoma cells. The fluorescence images showed that the PpIX distribute in cytoplasm. However the efficiency of ALA photodynamic inactivation to two cell lines was different. The leukemia cells were more sensitive for ALA-PDT than hepatoma cells, revealing that the ALA-PDT effect is cell line dependent. However by using ALA-Hexyl ester (He-ALA) instead of ALA, the cell photo-inactivation was improved. The PDT efficiency of He-ALA was 10 times high than that of ALA, showing He-ALA is a very promising drug in ALA-PDT.

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