Journal of Photoscience
- 제9권2호
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- Pages.308-310
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- 2002
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- 1225-8555(pISSN)
The Effect of S130A Mutant of pharaonis Halorhodopsin on Ability of Chloride Binding and Photocycle
- Sato, Maki (Division of Biological Sciences, Graduate School of Science, Hokkaido University) ;
- Kikukawa, Takashi (CAST, Hokkaido University) ;
- Araiso, Tsunehisa (CAST, Hokkaido University) ;
- Okita, Hirotaka (Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Shimono, Kazumi (Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Kamo, Naoki (Graduate School of Pharmaceutical Sciences, Hokkaido University) ;
- Demura, Makoto (Division of Biological Sciences, Graduate School of Science, Hokkaido University) ;
- Nitta, Katsutoshi (Division of Biological Sciences, Graduate School of Science, Hokkaido University)
- 발행 : 2002.08.01
초록
Bacteriorhodopsin (bR) and halorhodopsin (hR), which exist in the membrane of Halobacterium salinarum, are light-driven ion pumps. In spite of high similarity of primary and tertiary structures between bR and hR, these membrane proteins transport different ions, proton and chloride, in the opposite direction. From alignment of the amino acid sequences, Thr-89 of bR is homologous to Ser-l15 of hR from Halobacterium salinarum (shR). X-ray structure of shR has revealed that OH group of this residue directly interacts with CI