점액세균 Myxococcus sp. JW154로 부터 항균물질의 분리 및 식물병원균에 대한 in vivo 활성

Isolation and in vivo Activities of Antifungal Compounds from Myxococcus sp. JW154 (Myxobacteria).

  • 안종웅 (한국화학연구원 화학물질부) ;
  • 김병섭 (강릉대학교 식물응용과학과)
  • 발행 : 2002.06.01

초록

제주도 서귀포의 해변에서 채집한 해양퇴적물로부터 Myxococcus속의 점액세균 JW154를 분리하여 그 대사산물로부터 강한 항균활성을 나타내는 두 개의 bithiazole계 화합물을 분리하고 각종 기기분석을 통하여 구조를 확인한 바, 이들은 각각 KR025와 melithiazole F로 보고된 화합물임을 밝혔다. 유묘 검정법으로 조사된 이 화합물의 주요 식물병원균에 대한 in vivo 항균활성은 토마토 역병과 밀 녹병 및 보리 흰가루병에 대해 높게 나타났는데, 20$\mu\textrm{g}$/m1 수준으로 처리하였을 때 80% 이상의 높은 방제가를 보였다.

Two bithiazole-type antibiotics were isolated from the culture broth of a Myxococcus species which isolated from the marine sediment off the coast of Cheju Island, Korea. The structures of these metabolites were determined as KR025 and melithiazole F, previously reported bithiazoles, using combined spectroscopic methods. Both compounds showed an antifungal activity. In in vivo tests, these compounds exhibited potent controlling activities against tomato late blight, wheat leaf rust, and barley powdery mildew with control values more than 80% at a concentraton of 20 $\mu\textrm{g}$/ml.

키워드

참고문헌

  1. J. Nat. Prod. v.62 KR025, a new cytotoxic compound from Myxococcus fulvus Ahn, J.-W.;S. -H. Woo;C. -O. Lee;K. -Y. Cho;B. S. Kim https://doi.org/10.1021/np9804233
  2. Nature v.387 Cancer drugs better tan taxol? Cowden, C. J.;I. Paterson https://doi.org/10.1038/387238a0
  3. J. Antibiotics v.33 Myxothiazol, an antibiotic from Myxococcus fulvus Gerth, K.;H. Irschik;H. Reichenbach;W. Trowitzsch https://doi.org/10.7164/antibiotics.33.1474
  4. Canadian J. Microbiology v.15 Studies on the taxonomy of the Myxobacterales McCurdy, H. D. https://doi.org/10.1139/m69-259
  5. J. Antibiotics v.51 Cystothiazoles A and B, new bithiazole-type antibiotics from Cystobacter fulvus Ojika, M.;Y. Suzuki;A. Tsukamoto;Y. Sakagami;R. Fudou;T. Yoshimura;S. Yamanaka https://doi.org/10.7164/antibiotics.51.275
  6. Drug discovery from nature Myxobacteria as producers of secondary metabolites Reichenbach, H.;G. Hoefle
  7. TIBTECH v.6 Myxobacteria: a source of new antibiotics Reichenbach, H.;K. Gerth;H. Irschik;B. Kunze;G. Hosfle https://doi.org/10.1016/0167-7799(88)90100-X
  8. J. Antibiotics v.52 Melithiazoles, new β-methoxyacrylate inhibitors of the respiratory chain isolated from Myxobacteria Sasse, F.;B. Bohlendorf;M. Hermann;B. Kunze;E. Forche;H. Steinmetz;G. Hoefle;H. Reichenbach https://doi.org/10.7164/antibiotics.52.721
  9. Angew. Chem. Int. Ed. Engl. v.36 Epothilones: Promising natural products with taxol-like activity Wessjohhann, L. https://doi.org/10.1002/anie.199707151
  10. Kagakutoseibutsu v.27 Myxobacteria-Bacteria forming fruiting body Yamanaka, S.
  11. J. Gen. Appl. Microbiol. v.33 Isolation and identification of myxobacteria Yamanaka, S.;A. Kawaguchi;K. Komagata https://doi.org/10.2323/jgam.33.247
  12. Yamanaka, S., A. Kawaguchi, and K. Komagata. 1987. Isolation and identification of myxobacteria. J. Gen. Appl. Microbiol. 33: 247-265 https://doi.org/10.2323/jgam.33.247