• Title/Summary/Keyword: melithiazole F

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Isolation and in vivo Activities of Antifungal Compounds from Myxococcus sp. JW154 (Myxobacteria). (점액세균 Myxococcus sp. JW154로 부터 항균물질의 분리 및 식물병원균에 대한 in vivo 활성)

  • 안종웅;김병섭
    • Microbiology and Biotechnology Letters
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    • v.30 no.2
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    • pp.162-166
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    • 2002
  • Two bithiazole-type antibiotics were isolated from the culture broth of a Myxococcus species which isolated from the marine sediment off the coast of Cheju Island, Korea. The structures of these metabolites were determined as KR025 and melithiazole F, previously reported bithiazoles, using combined spectroscopic methods. Both compounds showed an antifungal activity. In in vivo tests, these compounds exhibited potent controlling activities against tomato late blight, wheat leaf rust, and barley powdery mildew with control values more than 80% at a concentraton of 20 $\mu\textrm{g}$/ml.

Isolation and Properties of Cytotoxic Antibiotics Produced by Myxococcus stipitatus JW150 (Myxococcus stipitatus JW150이 생산하는 세포독성 물질의 분리 및 특성)

  • 안종웅;이정옥
    • YAKHAK HOEJI
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    • v.46 no.2
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    • pp.108-112
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    • 2002
  • Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-glycoprotein. By employing a resistant subline of HCT15 to adriamycin (CL02), we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain JW150 was selected for study since an activity against CL02 cells was discovered in the strain. Cytotoxicity-guided fractionation of the culture broth led to the isolation of cystothiazole A and melithiazole F. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. as well as its morphological and physiological characteristics. Cystothiazole A and melithiazole F demonstrated potent cytotoxicity against certain human cancer cells with $IC_{50}$ values ranging from 0.03~ $0.72{\mu}{\textrm{g}}$/ml. Both compounds were interestingly as active against drug-resistant sublines CL02 and CP70 as against the corresponding parental cells.