Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Streptomyces sp. Producing New Polyene Antibiotics and In Vivo Antimicrobial Activity of Tetrin C Against Phytopathogenic Fungi

  • CHOI, WON-CHANG (Department of Clinical Pathology, Hyechun College) ;
  • SEOK-YEON HWANG (Department of Clinical Pathology, Chungbuk National University Hospital) ;
  • TAE-KYU PARK (Department of Life Science, College of Natural Science, Konkuk University) ;
  • SI-KWAN KIM (Department of Life Science, College of Natural Science, Konkuk University)
  • Published : 2002.04.01
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Abstract

A Streptomyces sp. isolated from a soil sample collected in Taejeon, Korea has previously been found to produce two new polyene antibiotics. The two new antibiotics were named "16-methyloxazolomycin (antibacterial)" and "tetrin C (antifungal)", and their chemical structures are presented elsewhere [10, 11]. In the current study, chemotaxonomy, numerical taxonomy, and ISP methods were all employed for the taxonomic study. The spore chains were spirales and the spore surface was smooth. The spore mass was a gray series and no melanin pigment was produced. On the basis of the morphological and physiological properties, the microorganism was identified to be Streptomyces erumpens, belonging to the gray series of category IV, as defined by Bergey′s Manual. Tetrin C at the concentration of 20 ${\mu}g$/ml demonstrated a potent in vivo (pot test) preventive effect against rice blast, rice sheath blight, cucumber gray mold, wheat powdery mildew, and barley leaf rust.

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References

  1. Calot, L. and A. P. Cercos. 1963. Streptomyces ornatus, nov. sp. et Streptomyces erumpens, nov. sp. producteurs dornamicine et antibiotique 117732. Ann. Inst. Pasteur (Paris) 105: 159-161
  2. Goodfellow, M. and G. Alderson. 1979. Numerical taxonomy of Actinomadura and related actinomycetes. J. General Microbiol. 112: 95-111 https://doi.org/10.1099/00221287-112-1-95
  3. Harper, J. J. and G. H. G. Davis. 1979. Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. International J. Systematic Bacteriol. 29: 56-58
  4. Hsu, S. C. and J. L. Lockwood. 1975. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Applied Microbiol. 29: 422-426
  5. ISCC-NBS Color-Name Charts Illustrated with Centroid Colors. National Bureau of Standards, U.S.A
  6. Kim, S. K., S. S. Kim, and C. H. Kim. 1990. New antibiotics produced by Streptomyces melanosporofaciens. I. Taxonomy of the producing microorganism. Kor. J. Appl. Microbiol. Biotechnol. 18: 624-632
  7. Mori, T., K. Takahashi, M. Kashiwabara, D. Uemura, C. Katayama, S. Iwadare, Y. Shizuri, R. Mitomo, F. Nakano, and A. Matsuzaki. 1985. Structure of oxazolomycin, a novel blactone antibiotic. Tetrahedron Lett. 26: 1073-1076
  8. Ogura, M., H. Nakayama, K. Furihata, H. Seto, and N. Otake. 1985. Structure of a new antibiotic curromycin A produced by a genetically modified strain of Streptomyces hygroscopicus, a polyether antibiotic producing organism. J. Antibiotics 38: 669-673
  9. Ogura, M., H. Nakayama, K. Furihata, A. Shimatsu, H. Seto, and N. Otake. 1985. Isolation and structural determination of a new antibiotic curromycin B. Agri. Biol. Chem. 49: 1909- 1910
  10. Ryu, G. S., S. J. Hwang, and S. K. Kim. 1997. 16- Methyloxazolomycin, a new antimicrobial and cytotoxic substance produced by a Streptomyces sp. J. Antibiotics 50: 1064-1066
  11. Ryu, G. S., W. C. Choi, S. J. Hwang, W. H. Yeo, C. S. Lee, and S. K. Kim. 1999. Tetrin C, a new glycosylated polyene macrolide antibiotic produced by Streptomyces sp. GK9244. J. Nat. Prod. 62: 917-919
  12. Shirling, E. B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. International J. Systematic Bacteriol. 16: 313-340
  13. Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. II. Species description from first study. International J. Systematic Bacteriol. 18: 69- 189
  14. Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. International J. Systematic Bacteriol. 18: 279-392
  15. Shirling, E. B. and D. Gottlieb. 1968. Cooperative description of type cultures of Streptomyces. IV. Species description from the second, third and fourth studies. International J. Systematic Bacteriol. 19: 391-512
  16. Shirling, E. B. and D. Gottlieb. 1972. Cooperative description of type strains of Streptomyces. V. Additional descriptions. International J. Systematic Bacteriol. 22: 265-394
  17. Waksman, S. A. 1959. Strain specificity and production of antibiotic substances. Proc. Nat. Acad. Sci. USA 45: 1043- 1047 https://doi.org/10.1073/pnas.45.7.1043
  18. Williams, S. T., M. E. Sharpe, and J. G. Holt. 1989. Bergey’s Manual of Systematic Bacteriology (Vol. 4). Williams & Wilkins, Baltimore, U.S.A
  19. Williams, S. T., M. Goodfellow, E. M. H. Wellington, J. C. Vickers, G. Alderson, P. H. A. Sneath, and M. J. Sackin. 1983. A probability matrix for identification of some Streptomyces. J. General Microbiol. 129: 1815-1830
  20. Williams, S. T., M. Goodfellow, G. Alderson, E. M. H. Wellington, P. H. A. Sneath, and M. J. Sackin. 1983. Numerical classification of Streptomyces and related genera. J. General Microbiol. 129: 1743-1813