Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of Lipase from the Anaerobic Lipolytic Bacterium Selenomonas lipolytica

  • Behere, AdiTi S. (Microbial Sciences Division, Agharkar Research Institute) ;
  • Dighe, Abhijit S. (National Centre for Cell Science, Pune University Campus) ;
  • Bhosale, Suresh B. (Microbial Sciences Division, Agharkar Research Institute) ;
  • Ranade, Dilip R. (Microbial Sciences Division, Agharkar Research Institute)
  • Published : 2002.02.01
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Abstract

Two different extracellular lipases were produced by an anaerobic bacterium, Selenomonas lipolytica. A major lipase, lipase I, was isolated, which showed optimum activity at pH 6.0 and at $45^{\circ}C$. It showed a molecular weight of 240 kDa and was a tetramer of a subunit having molecular weight of 60 kDa, which is different from the known bacterial lipases.

Keywords

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