Epidemiological Typing and Characterization of dfr Genes of Shigella sonnei Isolates in Korea During the Last Two Decades

  • Kim, Ki-Sung (Department of Microbiology, College of Medicine, Seonam University) ;
  • Oh, Jae-Young (Department of Microbiology, College of Medicine, Seonam University) ;
  • Jeong, Yong-Wook (Department of Microbiology, College of Medicine, Seonam University) ;
  • Cho, Jae-We (Department of Microbiology, College of Medicine, Seonam University) ;
  • Park, Jong-Chun (Department of Microbiology, College of Medicine, Seonam University) ;
  • Cho, Dong-Teak (Department of Microbiology, School of Medicine, Kyungpook National University)
  • 발행 : 2002.02.01

초록

One-hundred and twenty-four trimethoprim-resistant Shigella sonnei isolates extracted in Korea during the last two decades were investigated for their epidemiological relationship and mechanisms of resistance to trimethoprim. The S. sonnei isolates were distributed into two groups by three different epidemiological tools: biotyping, antibiogram, and pulsed-field gel electrophoresis. One group contained the isolates from the 1980s and the other group included the isolates from the 1990s. The geometric mean MICs of trimethoprim in S. sonnei isolates from the 1980s and 1990s were found to be $672.9{\mu}g/ml\;and\;>2,048{\mu}g/ml$, respectively. Trimethoprim resistance was associated with dfrA5, dfrA12, and dfrA13 genes in the isolates from the 1980s, dfrA1, dfrA5, and dfrA12 in the isolates from 1991, and dfrA1 and dfrA12 in the isolates from 1992 to 1999. The dfrA1 gene was located downstream of the intI2 gene in Tn7, which was located on chromosome. Some dfrA12 genes were found as gene cassettes in the class 1 integron. The dfrA5 and dfrA13 genes were located on conjugative plasmids. These results suggested that a clonal change occurred in S. sonnei isolates in Korea during the last two decades and that dfr genes located on different transposable genetic elements had gradually changed.

키워드

참고문헌

  1. Epidemiol. Infect. v.105 Molecular characterization of trimethoprim resistance in Shigella sonnei in Sicily Agodi, A.;C. Jones;E. J. Threlfall;M. D'Angelo;M. Marranzano https://doi.org/10.1017/S0950268800047610
  2. Biochem. Biophys. Res. Bommun. v.58 R-factor trimethoprim resistance mechanism: An insusceptible target site Amyes, S. G. B.;J. T. Smith https://doi.org/10.1016/0006-291X(74)90380-5
  3. Current Protocols in Molecular Biology Ausubel, F. M.;R. Brent;R. E. Kingston;D. D. Moore;J. G. Seidman;J. A. Smith;K. Struhl
  4. J. Bacteriol. v.125 Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistance from R483 to other replicons Barth, P. T.;N. Datta;R. W. Hedges;N. J. Grinter
  5. Nucleic Acids Res. v.7 A rapid alkaline extraction procedure for screening recombinant plasmid DNA Birnboim, I. C.;J. Doly https://doi.org/10.1093/nar/7.6.1513
  6. Escherichia, Shigella, and Salmonella(7th ed.) Manual of Clinical Microbiology Bopp, C. A.;F. W. Brenner;J. G. Wells;N. A. Strockbine;P. R. Murray(ed.);E. J. Baron(ed.);M. A. Pfaller(ed.);F. C. Tenover(ed.);R. H. Yolken(ed.)
  7. J. Infect. Dis. v.159 Dissemination of trimethoprim-resistant clones of Shigella sonnei in Bulgaria Bratoeva, M. P.;J. F. John Jr. https://doi.org/10.1093/infdis/159.4.648
  8. Antimicrob. Agents Chemother. v.43 Detection of a streptomycin/spectinomycin adenylyltransferase gene (aadA) in Enterococcus faecalis Clark, N. C.;Ø. Olsvik;J. M. Swenson;C. A. Spiegel;F. C. Tenover https://doi.org/10.1093/jac/43.1.157
  9. Nucleic Acids Res. v.11 The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harboured by Tn7 Fling, M. E.;C. Richards https://doi.org/10.1093/nar/11.15.5147
  10. Antimicrob. Agents Chemother. v.42 High-level resistance to trimethoprim in clinical isolates of Campylobacter jejuni by acquisition of foreign genes (dfr1 and dfr9) expressing drug-insensitive dihydrofolate reductases Gibrel, A.;O. Skold
  11. Epidemiol. Infect. v.104 Trimethoprim resistance gene in Shigella dysenteriae 1 isolates obtaines from widely scattered locations of Asia Haider, K.;A. Chatkaeomorakot;B. A. Kay;K. A. Talukder;D. N. Taylor;P. Echeverria;D. A. Sack https://doi.org/10.1017/S0950268800059380
  12. Drug Resist. Updates v.1 Antibiotic resistance in gram-negative bacteria: The role of gene cassettes and integrons Hall, R. M.;C. M. Collis https://doi.org/10.1016/S1368-7646(98)80026-5
  13. J. Infect. Dis. v.161 Increase of trimethoprim resistance among Shigella species, 1975-1988: Analysis of resistance mechanisms Heikkila, E.;A. Siitonen;M. Jahkola;M. Fling;L. Sundstrom;P. Huovinen https://doi.org/10.1093/infdis/161.6.1242
  14. Antimicrob. Agents Chemother. v.35 Analysis of genetic localization of the type 1 trimethoprim resistance gene from Escherichia coli isolated in Finland Hikkila, E.;L. Sundstrom;M. Skurnik;P. Huovinen https://doi.org/10.1128/AAC.35.8.1562
  15. Antimicrob. Agents Chemother. v.37 A novel dihydrofolate reductase cassette inserted in an integron borne on a Tn21-like element Heikkila, E.;M. Skurnik;L. Sundstrom;P. Huovinen https://doi.org/10.1128/AAC.37.6.1297
  16. J. Antimicrob. Chemother. v.25 The emergence and mechanisms of trimethoprim resistance in Escherichia coli isolated from outpatients in Finland Hikkila, E.;O. V. Renkonen;R. Sunila;P. Uurasmaa;P. Huovinen https://doi.org/10.1093/jac/25.2.275
  17. J. Microbiol. Biotechnol. v.10 Analysis of the genome of Symbiobacterium toebii by pulsed-field gel electrophoresis Hong, S. P.;J. H. Park;Y. S. Kim;H. J. Hwang;S. K. Rhee;S. G. Lee;M. H. Sung;N. Esaki
  18. Antimicrob. Agents Chemother. v.31 Trimethoprim resistance Huovinen, P. https://doi.org/10.1128/AAC.31.10.1451
  19. Antimicrob. Agents Chemother. v.39 Trimethoprim and sulfonamide resistance Huovinen, P.;L. Sundstrom;G. Swedberg;O. Skold https://doi.org/10.1128/AAC.39.2.279
  20. J. Antimicrob. Chemother. v.47 The prevalence of trimethoprim-resistance-conferring dihydrofolate reductase genes in urinary isolates of Escherichia coli in Korea Lee, J. C.;J. Y. Oh;J. W. Cho;J. C. Park;J. M. Kim;S. Y. Seol;D. T. Cho https://doi.org/10.1093/jac/47.5.599
  21. APMIS v.109 Antimicrobial resistance of Shigella sonnei in Korea during the last two decades Lee, J. C.;J. Y. Oh;K. S. Kim;Y. W. Jeong;J. W. Cho;J. C. Park;S. Y. Seol;D. T. Cho https://doi.org/10.1034/j.1600-0463.2001.090307.x
  22. J. Microbiol. Biotechnol. v.9 Antibacterial activity of antimycolic miconazole against methicillin-resistant Staphyolococcus aureus Lee, S. H.;C. J. Kim
  23. Nature v.297 Unique insertion site of Tn7 in the E. coli chromosome Liechtenstein, C.;S. Brenner https://doi.org/10.1038/297601a0
  24. Brazil. J. Clin. Microbiol. v.35 Molecular epidemiology of multiple antibiotic-resistant Shigella flexneri in Fortaleza Lima, A. A. M.;J. J. C. Sidrim;N. L. Lima;W. Titlow;M. E. Evans;R. N. Greenberg
  25. J. Clin. Microbiol. v.33 Analysis of clonal relationships among isolates of Shigella sonnei by different molecular typing methods Liu, P. Y.;Y. Z. Lau;B. S. Hu;J. M. Shyr;Z. Y. Shi;W. S. Tsai;Y. H. Lin;C. Y. Tseng
  26. Microbiol. Immunol. v.42 Epidemiologic study of Shigella sonnei from sequential outbreaks and sporadic cases using different typing techniques Matsumoto, M.;Y. Suzuki;M. Saito;N. Ishikawa;M. Ohta
  27. Epidemiol. Infect. v.110 rRNA gene restriction patterns and biotypes of Shigella sonnei Nastasi, A.;S. Pignato;C. Mammina;G. Giammanco https://doi.org/10.1017/S0950268800050640
  28. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aetobically(4th ed.) Approved standard M7-A4. National Committee for Clinical Laboratory Standards National Committee for Clinical Laboratory Standards
  29. Communicable Disease Monthly Report v.1 The frequency of shigellosis in Korea during the period 1985 to 1989 National Institute of Health in Korea
  30. Communicable Disease Monthly Report v.9 The frequency of shigellosis in Korea -1998. 8-10 National Institute of Health in Korea
  31. J. Clin. Microbiol. v.37 Typing and characterization of mechanisms of resistance of Shigella spp. isolated from feces of children under 5 years of age from Ifakara, Tanzania Navia, M. M.;L. Capitano;J. Ruiz;M. Vargas;H. Urassa;D. Schellemberg;J. Gascon;J. Vila
  32. J. Clin. Microbiol. v.32 Genetic variability and molecular typing of Shigella sonnei strains isolated in Canada Preston, M. A.;A. A. Borczyk
  33. Microbiol. v.141 Gene cassettes: A new class of mobile element Recchia, G. D.;R. M. Hall https://doi.org/10.1099/13500872-141-12-3015
  34. Molecular Cloning: A Laboratory Manual(2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  35. J. Microbiol. Biotechnol. v.11 Characterization of a cell line HFH-T2, producing viral particles, from primary human fetal hepatocytes in fected with Hepatitis B virus Shim, J. K.;D. W. Kim;T. H. Chung;J. K. Kim;J. I. Suh;C. Park;Y. C. Lee;T. W. Chung;E. Y. Song;C. H. Kim
  36. J. Bacteriol. v.155 Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: Comparison with prokaryotic and eukaryotic dihydrofolate reductases Simonsen, C. C.;E. Y. Chen;A. D. Levinson
  37. Antimicrob. Agents Chemother. v.27 Plasmid-borne or chromosomally mediated resistance by Tn7 is the most sommon response to ubiquitous use of trimethoprim Steen, R.;O. Skold https://doi.org/10.1128/AAC.27.6.933
  38. Antibiot. Chemother. v.9 Inocular replicating apparatus for routine testing of bacterial susceptibility to antibiotics Steers, E.;E. L. Flotz;B. S. Gravis;J. Riden
  39. Antimicrob. Agents Chemother. v.34 The dhfrl trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings Sundstrom, L.;O. Skold https://doi.org/10.1128/AAC.34.4.642
  40. Antimicrob. Agents Chemother. v.31 Novel type of plasmid-borne resistance to trimethoprim Sundstrom, L.;T. Vinayagamoorthy;O. Skold https://doi.org/10.1128/AAC.31.1.60
  41. J. Clin. Microbiol. v.33 Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing Tenover, F. C.;R. D. Arbeit;R. V. Goering;P. A. Mickelsen;B. E. Murray;D. H. Persing;B. Swaminathan
  42. Antimicrob. Agents Chemother. v.42 Class 1 integron-borne multiple-antibiotic resistance carried by IncFI and IncL/M plasmids in Salmonella enterica serotype Typhimurium Tosini, F.;P. Visca;I. Luzzi;A. M. Dionisi;C. Pezzella;A. Petrucca;A. Carattoli
  43. J. Antimicrob. Chemother. v.33 Genetic structures associated with spread of the type Ia trimethoprim-resistant dihydrofolate reductase gene amongst Escherichia coli strains isolated in the Nottingham area of the United Kingdom Towner, K. J.;A. Brennan;Y. Zhang;C. A. Holtham;J. L. Brough;G. I. Carter https://doi.org/10.1093/jac/33.1.25
  44. Antimicrob. Agents Chemother. v.38 Antimicrobial resistance of Shigella isolates causing traveler's diarrhea Vila, J.;J. Gascon;S. Abdalla;J. Gomez;F. Marco;A. Moreno;M. Corachan;T. Jimenez de Anta https://doi.org/10.1128/AAC.38.11.2668