Molecular Marker Analysis for Resistance of Soybean Cultivars to Soybean Cyst Nematode

  • Chung, Jong-Il (Department of Agronomy, Gyeongsang National University) ;
  • Park, Won-Gyeong (Department of Agronomy, Gyeongsang National University) ;
  • Park, Min-Jung (Department of Agronomy, Gyeongsang National University) ;
  • Ko, Mi-Suk (Department of Agronomy, Gyeongsang National University)
  • 발행 : 2002.09.01

초록

Soybean cyst nematode (Heterodera glycines Ichinohe; SCN) is an important soybean pest and the use of resistant cultivars is the effective method to reduce or eliminate SCN damage. However, breeding for SCN resistance is difficult and expensive by the oligogenic nature of the resistance and genetic variability in the pathogen. Fortunately, SCN resistance loci, rhg1 and Rhg4 are generally accepted as a necessity for the development of resistant genotypes using any source of resistance. In this study, resistance of 44 Korean soybean cultivars to SCN was tested using two molecular markers. Seonheukkong and Pokwangkong were the homozygous to rhg1 locus. Seven cultivars were susceptible to SCN based on Satt309 marker linked rhg1 locus. All Korean cultivars estimated in this study were recessive homozygous to Rhg4 locus and were susceptible in the PCR reaction using primer 548/563 linked to the Rhg4 locus conferring resistance to SCN race 3. Among 44 cultivars estimated, seven cultivars were susceptible to SCN in both Satt309 and primer 548/563 markers. Based on both Satt309 and primer 548/563 markers, there is no resistant cultivar to SCN in Korea. Therefore, SCN resistant cultivars need to be developed in the future. These two markers can be used for improving SCN resistant cultivars.

키워드

참고문헌

  1. Akkaya, M. A., A. Bhagwat, and P.B. Cregan. 1992. Length polymorphism of simple sequence repeat DNA in soybean. Genetics 132:1131-1139
  2. Caldwell, B. E., C. A. Brim, and J. P. Rose. 1960. Inheritance of resistance of soybeans to the cyst nematode, Heterodera gly-cines. Agron. J. 52 : 635-636 https://doi.org/10.2134/agronj1960.00021962005200110007x
  3. Caviness, C. E. 1992. Breeding for resistance to soybean cyst nematode. In: Riggs RD, Wrather JA (eds) Biology and management of the soybean cyst nematode. APS press, st. Paul, Minn., pp. 143-156
  4. Condbido, V. C., R. L. Denny, S. R. Boutin, R. Hautea, J. H. Orf, and N. D. Young. 1994. DNA marker analysis of loci underly-ing resistance to soybean cyst nematode (Heterodera gtycines Ichinohe). Crop Sci 34 : 240-246 https://doi.org/10.2135/cropsci1994.0011183X003400010044x
  5. Concibido, V. C., R. L. Denny, D. A. Lange, J. H. Orf, and N. D. Young. 1996. RFLP mapping and Marker-assisted selection of soybean cyst nematode resistance in PI209332. Crop Sci 36 : 1643-1650 https://doi.org/10.2135/cropsci1996.0011183X003600060038x
  6. Concibido, V. C., D. A. Lange, R. L. Denny, J. H. Orf, and N. D. Young. 1997. Genome mapping of soybean cyst nematode resistance genes inpeking, PI90763, PI88788 using DNA markers. Crop Sci 37: 258-264 https://doi.org/10.2135/cropsci1997.0011183X003700010046x
  7. Cregan, P. B., T. Jarvik, A. L. Bush, R. C. Shoemaker, K. G. Lark, A. L. Kahler, N. Kaya, T. T. VanToai, D. G. Lohnes, J. Chung, and J. E. specht. 1999. An integrated genetic linkage map of the soybean genome. Crop Sci. 39 : 1464-1490 https://doi.org/10.2135/cropsci1999.3951464x
  8. Cregan, P. B., J. Mudge, E. W. Fickus, D. Danesh, R. Dermy, and N. D. Young. 1999. Two simple sequence repeat markers to select for soybean cyst nematode resistance conditioned by the rhgl locus. TheorAppl Genet. 99 : 811-818 https://doi.org/10.1007/s001220051300
  9. Kim, D. G., J. K. Lee, and Y. K. Lee. 1999. Distribution of Races of Soybean Cyst Nematode in Korea. Korean J. Appl. Entomol. 38(3): 249-253
  10. Matson, A. L., and L. F. Williams. 1965. Evidence of a fourth gene for resistance to the soybean cyst nematode. Crop Sci. 5 : 477 https://doi.org/10.2135/cropsci1965.0011183X000500050032x
  11. Myers, G. 0., and S. C. Anand. 1991. Inheritance of resistance and genetic relationships among soybean plant introductions to races of soybean cyst nematode. Euphytica. 55 : 197-201 https://doi.org/10.1007/BF00021239
  12. Mahalingam, R., and H. T. Skompska. 1995. DNA markers for resistance to Heterodera glycines race3 in the soybean cultivar peking. Breed. Sci. 45 :435-443
  13. Matthews, B. F., M. H. MacDonald, J. D. Gebhart. and T. E. Devine. 1998. PCR markers residing close to the Rhg4 locus conferring resistance to the soybean cyst nematode race3 on linkage gorup $A_2$ of soybean. Theor Appl Genet. 97 : 1047-1052 https://doi.org/10.1007/s001220050990
  14. Niblack, T. L., and D. C. NOrton. 1992. Soybean yield losses due to Heterodera glycines in lowa. Plant Dis 76 : 943-948 https://doi.org/10.1094/PD-76-0943
  15. Riggs, R. D., and D. P. Schmitt. 1988. Complete characterization of the race scheme for Heterodera glycines, J. Nematol. 20 : 392-395
  16. Rao-Arelli, A. P, S. C. Anand, and J. A. Wrather. 1992. Soybean resistance to soybean cyst nematode Race 3 is conditioned by an additional dominant gene. Crop Sci. 32 : 862-864 https://doi.org/10.2135/cropsci1992.0011183X003200040005x
  17. RaoArelli, A. P. 1994. Inheritance of resistance to Heterodera sty-cines Race 3 in soybean accesions. Plant Dis 78 : 898-900 https://doi.org/10.1094/PD-78-0898
  18. Williams, J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA polymorphisms amplified by a arbi-trary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535 https://doi.org/10.1093/nar/18.22.6531
  19. Webb, D. M., B. M. Baltazar, A. P. Rao-Arelli, J. Schupp, P. Kelim, K. Clayton, A. R. Ferrira, T. Owens, and W. D. Beavis. 1995. Genetic mapping of soybean cyst nematoce race3 resis-tance loci in the soybean PI437654. Theor Appl Genet 91 : 574-581