Effect of Light Source on Organic Acid, Sugar, and Flavonoid Concentrations in Buckwheat

  • Kim, Sun-Lim (National Crop Experiment Station, RDA) ;
  • Lee, Han-Bum (Gyonggi Provincial Agricultural Research and Extention Services) ;
  • Park, Cheol-Ho (College of Agricultural and Life Sciences, Kangwon National University)
  • Published : 2002.03.01

Abstract

The major free sugars of buckwheat plants were fructose, glucose, and maltose but their contents and compositions were influenced by the different wavelength of light. Free sugar contents of Clfa 39 (Fagopyrum tataricum) were higher than those of Yangjul-maemil (Fagopyrum esculentum) regardless of the light sources. As treated with red and blue light, the free sugar contents in the leaves of buckwheat plants were slightly increased, but their contents in the stems and flowers were lower than those of natural light condition. Under the natural light condition, maltose was detected in every tissues of buckwheat plants, but as treated with blue and red light, it was not detected in the flowers of buckwheat plants. Citric, malic and acetic acid were detected as major organic acids in buckwheat plants. Red and blue lights decreased the total organic acid contents in buckwheat plants as compared with natural light condition. It was considered that blue light are less active than red light for the accumulation of organic acids. Tataric acid was detected only in the leaves of buckwheat plants, however, as treated with red and blue light, it was not detected in the leaves of Clfa 39. Flowers of Yangjul-maemil contained a considerable amount of rutin and quercitrin. Only small amount of quercitrin was detected in leaves, but it was not detected in stems. On the other hand, Clfa 39 leaves contained a considerable amount of rutin, quercetin and small amount of quercitrin, but quercitrin and quercetin were detected only in the stems of Clfa 39. Red and blue lights significantly decreased the contents of rutin, quercitrin, and quercetin in buckwheat plants as comparing with natural light condition. Rutin content in the flowers of Clfa 39 was increased under the red and blue light conditions.

Keywords

References

  1. Amrhein N. 1979. Biosynthesis of cyanidin in buckwheat hypocotyls. Phytochemistry 18(4): 585-589 https://doi.org/10.1016/S0031-9422(00)84265-X
  2. Avigad G. 1993. Disaccharides. In : Dey P. M. and J. B. Hasrbome. 1993. Methods in plant biochemistry. VoL 2. Carbohydrates : pp. 115-121
  3. Couch J.F., J. Naghski, and C. F. Krewson. 1946. Buckwheat as a source of rutin. Science(Februaiy): 197-198
  4. Drew, E. A., 1983. Sugars, cyclitols and seagrass phylogeny. Aquat. Bot. 15 : pp. 387-408 https://doi.org/10.1016/0304-3770(83)90007-4
  5. Hagels H., W. Dietmar and S. Heinz. 1995. Phenolic compounds of buckwheat herb and influence of plant and agricultural factors (Fagopyrum escuIentum Moench and Fagopyrum tataricum Gartner. Current advances in buckwheat research : pp.801-809
  6. Harper D. B. H., J. A. Douglas and H. Smith. 1970. The photocontrol of precursor incorporation into the Pisum sativum flavonoids. Phytochemistry. 9(3) : 497-505 https://doi.org/10.1016/S0031-9422(00)85680-0
  7. Hart J. W. 1988. Light and plant growth. Unwin Hyman, London : pp. 3-7, 30,71-73,96-97
  8. Havsteen B. 1983. Flavonoids, a class of natural products of high pharrnacological potency. Biochemical pharmacology. 32(7) :1141-1148 https://doi.org/10.1016/0006-2952(83)90262-9
  9. Kim S. L., C. H. Park, E. H. Kim, H. S. Hur, and Y. K. Son. 2000. Physicochemical characteristics of corn silk. Korean J. Crop Sci. 45(6): 392-399
  10. Kim, S. L., Y. K. Son, J. J. Hwang, S. K. Kim, and H. S. Hur. 1998. Development of buckwheat sprouts as a functional vegetable. RDA.J.Crop Sci.: 191-199
  11. Quettier-Deleu C., B. Gressier, J. Vasseur, T. Dine, C. Brunet, M. Luyckx, M. Cazin, J. C. Cazin, F. Bailleul, and F. Trotin. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. of Ethnophannacology. 72(1-2): 35-42 https://doi.org/10.1016/S0378-8741(00)00196-3
  12. Lewis C. E., J. R. L.Walker, J. E. Lancaster and A. J. Conner. 1998. Light regulation of anthocyanin, flavonoid and phenolic acidbiosynthesis in potato minitubers in vitro. Australian J. of Plant Physi. 25(8): 915-922 https://doi.org/10.1071/PP98112
  13. Mohr H. and P. Schopfer. 1995. Plant physiology. Springer : pp. 177-200,206-207, 275-283
  14. Smith H. and T. H. Attridge. 1970. Increased phenylalanine ammonia-lyase activity due to light treatment and its significance for the mode of action of phytochrome. Phytochemistry. 9(3): 487-495 https://doi.org/10.1016/S0031-9422(00)85679-4
  15. Smith H. and D. B. Harper. 1970. The effect of short- and longterm irradiation on the flavonoid complement of the terminal buds of Pisum sativum var. Alaska. Phytochemistry. 9(3): 477-485 https://doi.org/10.1016/S0031-9422(00)85678-2
  16. Tomasko, D. A., 1993. The physiological basis for responses to light availability, In: Morris, L. J. and Tomasko, D. A., Editors, 1993. Proceedings and conclusions of workshops on the submerged aquatic vegetation initiative and photosyntheticaIIy active radiation. Special Publication SJ93-SP13, St. Johns River Water Management District, Palatka, FL : pp. 211-218