Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Cytotoxic Activities and Antioxidative Activities Against Liver Cancer Cell of Albizzia root

합환근의 항산화효과와 간암세포에 대한 세포독성

  • 강병수 (동국대학교 한의과 대학 본초학교실) ;
  • 이갑득 (동국대학교 자연과학대학 화학과)
  • Published : 2002.12.01
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Abstract

To find new inhibitory effects from oriental drugs, Albizziae root was extracted in methanol and the extracted was stepwisely fractionated by hexane, chloroform, ethylacetate, butanol and water. In cytotoxic effect of Albizziae root fractions against cancer cell lines including human hepatoma cells(HepG2) were investigated. Expecially the butanol fraction exhibited a inhibition effects on the growth of human hepatoma cells(HepG2). It inhibited of HepG2 cells with the value of IC50. The activities of qutathione after B(a)P treatment were markedly decreased than control, but those levels were increased by the treatment of Albizziae root methanol fraction. The activity of glutathione-S-transferase after B(a)P treatment were markedly decreased than control, but those levels were increased by the treatment of Albizziae root methanol traction. Induction of phase II enzymes is a major mechanism of chemoprevention. The induction levels of quinone reductase(QR) activity in cultured murine hepatoma(Hepa IcIc7)cell by methanol extract of Albizziae root were measured. Among the tested tractions, the extracts of butanol were found to induce QR activities over 2.8 fold than control. These results suggest that Albizziae root has chemopreventive Potential by inducing QR activities and GST levels and increasing GSH

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References

  1. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analyt. Biochem., 72, 248 https://doi.org/10.1016/0003-2697(76)90527-3
  2. Boyland, E. and Chasseud, C.F. (1979). The role of Glutathione and Gkitathione S-transferase in mercaptureic acid biosynthesis, Adv. Cancer Res., 29, 175
  3. Benson, A.M., Hunkeler, M.J. and Talalay, P. (1980). Increase of NAD(P)H: Quinone reductase by dietary antioxidants; Possible role in protection against carcinogenesis and toxicity. Proc. Natl. Acad. Sci. U.S.A., 77, 5216 https://doi.org/10.1073/pnas.77.9.5216
  4. Chang J.S., Kim H.J., Bae J.T., Park S.H., Lee, S.E., Kim O.M. and Lee K.R. (1998). Inhibition effects of Auricularia auricula-judae methanol extract on lipid peroxidation and liver damage in benzo(a)Pyrene treated mice. J. Korean Soc. Food Sci. Nutr., 27(4), 712
  5. Doll, R. (1992). The lessons of life. Cancer Res., 52, 2024S-2029
  6. Elltnan, G. L. (1959). Tissue sulfhydryl group. Arch. Biochem Biophy., 82, 70 https://doi.org/10.1016/0003-9861(59)90090-6
  7. Green, L.M., Reade, J.L. and Ware, C.F. (1984). Rapid colometric assay for cell viability: Application to the quantitation of cytotoxic and growth inhibitory lymphokines. J. Immunological Methods, 70, 257 https://doi.org/10.1016/0022-1759(84)90190-X
  8. Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974). Glutathione S-transferase: The first enzymatic step in mercapturic acid reaction. Anal. Biochem., 249(22), 7130
  9. Molders, P. and Jernestion, B. (1983) Interaction of glutathione wiith reactive intermediates. In Larson, A. Orrcnius, S., Holgren, A and Mannervik, B. (eds.), Functions of glutatione: biochemical, Physiological, toxicological, and clinical aspects. 99-108. Reven Press, New York, NY
  10. Mitchell, J.R., Hinson, J.A. and Nelson, S.D. (1976) Glutathione and drug induced tissue lesions: metabolism and function. In Arias, I.M. and Jakoby, W.B. (eds), Glutathione, 357-367. Raven Press, New York, NY
  11. Sakamoto, Y. and Kino S. (1988). Glulathioncの 生理活性. In 'Glutathione'. 3rd ed, 講談社, 東京 5
  12. Talalay, P., DeLong, M.J. and Prochaska, H.J. (1987). cCory. J.G., and Szentivani, A., eds, Plenum, New York, p. 197
  13. Tanaka, T., T. Kojima, A. Hara, H. Sawada and H. Mori. (1993). Chemoprevention of oral carcinogenesis by D.L-difluoromethylornmithine, an ornithine decarboxylase inhibitor: dose-dependent reduction in 4-nitroquino1ine l-oxide-induced tongue neoplasmas in rats. Cancer Res., 53, 772-776
  14. Wattenberg, L.W. (1985). Chcmoprevention of cancer: Cancer Res., 45. 1
  15. Wattenbcrg, L.W. (1997). An overview of chemoprevention: current state and future propects. Proc. Soc. Exp. Biol. Med., 216, 133-141 https://doi.org/10.3181/00379727-216-44163
  16. 김명주(1991). 鹿角이 Benzo(a)Pyrene에 의해 유도된 간장해에 미치는 영향, 영남대학교 석사학위논문
  17. 최병돈, 염곤(2000). 국내 생악지원으로부터의 항종양효과의 검색. 생약학회지, 31(1), 16-22
  18. 呉普 述著(1994). 神最本草經, 醫聖堂, 서울, 권2, 26-27
  19. 李時珍(1982). 本草綱目, 人民衛生出版社, 北京 2013-2014
  20. 顔正華 主編(1991). 中藥擧, 人民術生出版祉, 北京 669-670
  21. 李京淳(1986). Albizzia julibrissin Durazz 엑기스의 抗眞菌作用, 忠北大榮擧論丈 隻 1,172
  22. 李盛雨(2000). 歡皮 抽出物이 마우스의 肝毒性 抑制敎果 및 癌細胞 成長 沮害效이果에 관한 硏究, 東國大學敎 大學院 韓醫擧科
  23. 主田成記, 性藤淸美(1988). Glutathione S-transferase isozyme, glutathione 硏究の工ボツク.榮白質, 核酸, 酵素,臨時增 刊, 33, 1370
  24. 배송자, 김남홍, 고진복, 노승배, 정복미(1997). 고들빼기 식이가 간 독성을 유발한 흰쥐의 효소 활성에 미치는 영향. 한국영앙학회지 , 30, 19-24
  25. 김선여, 김홍표, 이미경, 변순정, 김승희, 문애리, 한형미, 허훈, 김영중(1993). 사염화탄소에 의하여 유발된 흰주의 간독성에 미치는 비데인의 효과. 약학회지 37(5), 538-543