Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Developmental Immunotoxicity in SD Rat Pups Exposed by Di(n-butyl) Phthalate through Pre and Postnatal

SD Rat에 있어서 출생 전.후에 걸친 Di(n-butyl) Phthalate 노출에 의한 발생면역독성

  • 엄준호 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 정승태 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 이종권 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 박재현 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 권태우 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 김지영 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 오혜영 (식품의약품안전청, 국립독성연구소 독성부) ;
  • 김형수 (식품의약품안전청, 국립독성연구소 독성부)
  • Published : 2002.12.01
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Abstract

Phthalate esters have possible effects on the endocrine system. Di-n-butyl phthalate (DBP) is one of the most commonly wed phthalic acid esters (PAEs). It is extensively wed as a plasticizer in elastomers, as a solvent for printing inks and resins, and as a textile lubricating agent. It is also present in the formulations of various cosmetic products. DBP has been identified as a reproductive toxicant in several animal species and also know as a endocrine disruptor. The objective of this study was to investigate the effect of DBP on developmental immune Junction wing rat pups as experimental animals. Timed-bred pregnant SD rats were orally dosed with 0, 250, 500, or 750 mg DBP/kg body weight once a day from gestational day (GD) 5 to 18 and postpartum day (PD) 3 to 18. On PD22, the dams and their pups were euthanized and examined for alteration in parameters associated to immune function. The results showed no significant changes in body weight, thymus weight, thymus and spleen cellularities, the polyclonal activation respones of splenocyte with ConA and LPS, and also the distribution of arterial blood cells and thymocyto subsets in both rat dam and pups. However DBP exposure on rat dam resulted in increases of liver weights of dam and their pups except 750 mg DBP/kg, and body and spleen weights in pups except 750 mg DBP/kg. On the other hands, distribution rates of CD8+ T cells at 500 mg DBP/kg and B cells at 750 mg DBP/kg among splenocyte subsets were significantly increased in rat pups, unlike dams. Reasons of these distribution alterations of CD8+ T cells and B cells in rat pups are under study.

Keywords

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