The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
권호 표지
DOI QR코드

DOI QR Code

First Report of Tobacco mild green mosaic virus Infecting Pepper in Korea

  • Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jae-Hyun (National Horticultural Research Institute, Rural Development Administration) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University) ;
  • Choi, Jang-Kyung (Division of Biological Environment, Kangwon National University) ;
  • Chae, Soo-Young (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration) ;
  • Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Hyun-Ran (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Yong-Mun (National Horticultural Research Institute, Rural Development Administration)
  • 발행 : 2002.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A rod-shaped virus was isolated from pepper showing mild mosic during the winter growing seasons of 2001 and 2002 in Korea. Based on its biological reactions, serological relationships, reverse transcription-poly-merase chain reaction (RT-PCR) using specific primers, and nucleotide sequence analysis of coat protein (CP) gene, the isolated virus was identified as Tobacco mild green mosaic virus (TMGMV) and designated as Korean pepper isolate (TMGMV-KP). Crude sap from infected tissue was mechanically transmitted to various indicator plants, which produced characteristic symptoms of tobamovirus infection. However, no symptom was observed in Gomphorena globosa. In RT-PCR assays with specific primers toy respective detection of TMGMV, Tobacco mosaic virus (TMV), Pepper mild mottle virue (PMMoV), and Tomato mosaic virus (ToMV), a single strong band of about 500 bp in length was produced from the sample used only with TMGMV primers. The amplified DNA was cloned and the nucleotide sequence was determined. Sequence comparisons with the CP gene of other tobamoviruses indicated that TMGMV-KP shared 99.3％ identity with TMGMV Japanese isolate and only 59.1, 58.6, and 58.1％ identity with TMV, PMMoV and ToMV, respectively. This is the first report of TMGMV in Korea.

키워드

참고문헌

  1. Choi, G. S., Kim, J. H., Chung, B. N., Kim, H. R. and Choi, Y. M. 2001. Simultaneous detection of three Tobamoviruses in cucurbits by rapid immunofilter paper assay. Ptant Pathot. J. 17:106-109
  2. Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Korean J. Plant Pathol. 14:7-12
  3. Choi, J. K., Park, Y. S., Kim, J. O. and Park, E. K. 1989. Biologi-cal characterization of a strain of tobacco mosaic virus isolated from red pepper. Korean J. Plant Pathol. 5:331-336
  4. Cohen, J., Rosner, A., Kagan, S., Lampel, S., Beckelman, H., Maslenin, L., Zeidan, M. and Gera, A. 2002. A New disease in Tabemamontana caused by Tobacco mild mosaic virus. Acta Hort. 568:103-109
  5. Frail, A., Malpica, J. M., Aranda, M. A., Rodriguez-Cerezo, E. and Gracia-Arenal, F. 1996. Genetic diversity in tobacco mild green mosaic tobamovims infecting the wild plant Nicotiana gtauca. ViroIogy 223:148-155
  6. Gibbs, A. J. 1988. Tobacco mild green mosaic virus. Plant Viruses Online: Descriptions and Lists from the VIDE Database
  7. Gooding, G. V. and Hervert, 1967. A simple technique for purifi-cation of tobacco mosaic virus in large quantities. Phytopa-thology57:1285
  8. Kamer, G. and Argos, P. 1984. Primary structural comparison of RNA-dependent polymerase from plant, animal and bacterial virus. Nucteic Acids Res. 12:7269-7282 https://doi.org/10.1093/nar/12.18.7269
  9. Kim, J. H., Choi, G. S. and Choi, J. K. 2002. Characterization of Cucumber mosaic virus subgroup II isolated from paprika (Capsicum annuum var. grossum) in Korea. Plant Pathol. J. 18:6-11 https://doi.org/10.5423/PPJ.2002.18.1.006
  10. Kim, J. S., Kim, S. K., Choi, G. S. and Lee, M. W. 1990. Virus disease incidence and symptom appearance in red pepper. Korean J. Plant Pathol. 6:125-132
  11. Knorr, D. A. and Dawson, W. O. 1988. A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensi-tivity in Nicotiana sylvestris. Proc. Natt. Acad. Sci. U.S.A. 85:170-174 https://doi.org/10.1073/pnas.85.1.170
  12. Laemmli, U: K. and Favre; M. 1973. Maturation of the head of bacteriophage T4. I. DNA packaging event. J. Mot. Biol.80:575-599 https://doi.org/10.1016/0022-2836(73)90198-8
  13. Leonard, D. A. and Zaitlin, M. 1982. A temperature-sensitive strain of tobacco mosaic defective in cell-to-cell movement generates an altered viral-coded protein, Virology 117:416-424 https://doi.org/10.1016/0042-6822(82)90480-9
  14. McKinney, H. H. 1935. Evidence of virus mutation in the com-mon mosaic of virus. J. Agric. Res. 39:557
  15. Ouchterlony, O. 1968. Handbook of immunodiffusion and immu-noelectrophoresis. Prog. Allergy. Ann. Arbor Sci. Pubt. p. 135
  16. Satio, T., Meshi, T., Takamatsu, N. and Okada, Y. 1987. Coat protein gene sequence of tobacco mosaic virus encodes a host response determinant. Proc. Natl. Acad. Sci. U.S.A. 84:139-144
  17. Solis, I. and Garcia-Arenal, F. 1990. The complete nucleotide sequence of the genomic RNA of the tobamovims tobacco mild green mosaic Virus: Virology 177:553-558 https://doi.org/10.1016/0042-6822(90)90520-2
  18. Van Regenmortel, M. H. V. and Fraenkel-Conrat, H. 1986 Tobacco mosaic virus, In: The Ptant Viruses, pp. 79-100, Plenum Press, New York
  19. Watterson, J. C. 1993. Development and breeding of resistance to pepper and tomato viruses, In: Resistance to Virat Diseases of Vegetables, ed. by Kyle, M. M. pp. 80-101, Timber Press, New York

피인용 문헌

  1. Complete genome sequence supports Bell pepper mottle virus as a species of the genus Tobamovirus vol.152, pp.7, 2007, https://doi.org/10.1007/s00705-007-0950-0
  2. Development of Single-tube Multiplex Immunocapture RT-PCR Assay for Simultaneous Detection of Two Pepper Tobamoviruses vol.22, pp.2, 2006, https://doi.org/10.5423/PPJ.2006.22.2.164
  3. Tobacco mild green mosaic virus in Impatiens and Osteospermum: new hosts and first report in the UK vol.59, pp.6, 2010, https://doi.org/10.1111/j.1365-3059.2010.02338.x
  4. CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.) vol.31, pp.6, 2013, https://doi.org/10.7235/hort.2013.13021
  5. Molecular evidence supporting the confirmation of Maracuja mosaic virus as a species of the genus Tobamovirus and production of an infectious cDNA transcript vol.151, pp.12, 2006, https://doi.org/10.1007/s00705-006-0823-y
  6. Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (Impatiens balsamina) vol.154, pp.5, 2009, https://doi.org/10.1007/s00705-009-0377-x