Journal of Plant Biotechnology

  • 제29권1호
  • /
  • Pages.45-49
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  • 2002
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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털머위 (Farfugium japonica)의 캘러스 유도 및 식물체 분화에 미치는 생장조절제의 영향

Effect of Plant Growth Regulators on Callus Induction and Plant Regeration of Farfugium japonica

  • 이승엽 (원광대학교 식물자원과학부 생명자원과학연구소) ;
  • 유성오 (원광대학교 식물자원과학부 생명자원과학연구소) ;
  • 배종향 (원광대학교 식물자원과학부 생명자원과학연구소) ;
  • 이중호 (원광대학교 식물자원과학부 생명자원과학연구소)
  • Lee, Seung-Yeob (Institute of Life Science and Natural Resources, Division of Plant Resources Science, Wonkwang University) ;
  • Yoo, Sung-Oh (Institute of Life Science and Natural Resources, Division of Plant Resources Science, Wonkwang University) ;
  • Bae, Jong-Hyang (Institute of Life Science and Natural Resources, Division of Plant Resources Science, Wonkwang University) ;
  • Lee, Joong-Ho (Institute of Life Science and Natural Resources, Division of Plant Resources Science, Wonkwang University)
  • 발행 : 2002.03.01
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초록

털머위의 조직배양에 의한 식물체의 기내 미세증식을 위하여, 잎과 잎자루 조직으로부터 캘러스 유도 및 식물체 분화에 미치는 몇 가지 생장조절제들의 영향을 조사하였다. 캘러스 유도 및 생장은 잎과 잎자루 조직 모두 1∼2 mg/L 2,4-D와 1∼2 mg/L BA의 혼합배지에서 양호하였으며, 치상 조직간 캘러스 유도율은 비슷하였으나, 캘러스 형성시기는 잎절편에서 보다 잎자루 조직에서 더 빠르고 왕성하였다. 캘러스로부터 식물체 분화를 위한 적정 생장조절제의 농도는 1 mg/L NAA와 2 mg/L BA 혼합배지였다. 분화된 식물체는 캘러스와 함께 동일배지에 60일 정도 계대배양하면 다아체를 형성하였다. 분화된 유식물체는 0.5 mg/L IAA를 첨가한 MS배지에 옮겨 30일간 생육시킨 후, 초장 50 mm 이상의 소식물체를 vermiculite에서 순화시켰을 때 생존율은 95% 이상으로 높았으며, 토양활착도 잘 되었다.

The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.

키워드

참고문헌

  1. Ahn JC, Hwang SJ, Hwang B (2000) Micropropagation of some medicinal plants and production of valuable secondary metabolites by organ culture. Kor J Medicinal Crop Sci 8:12-25
  2. Cho YH, Park EA, Chiang MH (2000) Effects of nitrogen form of nutrient solution on the growth of Aster tataricus, Chrysan-themum. boreaIe, and Farfugium japonicum. Kor J Hort Sci Technol 18:14-17
  3. Choi JY, Kim HJ, Hyung NI (1998) Plant regeneration via organogenesis from leaf and stipule segments of strawberry (Fragaria ananassa Duch.). Kor J Plant Tiss Cult 25:347-351
  4. Hachey JH, Shama KK, Moloney MM (1991) Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Rep 9:549-554
  5. Kaul V, Miller RM, Hutchinson JF, Richards D (1990) Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelez (syn. Crysanthemum moIifolium Ramat.). Plant Cell Tiss Org Cul 21:21-30 https://doi.org/10.1007/BF00034487
  6. Kim MS, Park KW (1987) Effect of plant growth regulators on organ differentiation as cultivars and organs of sweet potato (Ipomoea batatas Poir.) by in vitro. J Kor Soc Hort Sci 28:233-244
  7. Kim TJ (1996) Korean resources plants. Seoul Natl Univ Press, Seoul
  8. Kim TS, Park MS, Park HK, Kim S, Jang YS (1994) Plant regeneration and in vitro tuber enlargement from callus in PineIlia ternata. (Thunb.) Breit. Kor J Medicinal Crop Sci 2:146-250
  9. Lee Jl, Park YH, Park YS, Park RK (1985) Tissue cultural studies on the maintenance and multiplication of parental lines in rape (Brassica napus L.) heterosis breeding. III. Effects of growth regulators on multiple shoots differentiation in stem culture of rape by in vitro. Kor J Breed 17:152-157
  10. Lu C, Nugent G, Wardley T (1990) Efficient, direct plant rege-neration from stem segments of Crysanthemum (Crysanthemum moIifolium Ramat.). Plant Cell Rep 8:733-736 https://doi.org/10.1007/BF00272106
  11. Matsubara S, Hegazi HH (1990) Plant regeneration from hypocotyle callus of radish. Hort Sci 25:1286-1288
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Nehra NS, Stushnoff C, Kartha KK (1989) Direct shoot rege-neration from strawberry leaf disks. J Amer Soc Hort Sci 114:1014-1018
  14. Nehra NS, Stushnoff C, Kartha KK (1990) Regeneration of plants from immature leaf-derived callus of strawberry (Fragaria ananassa). Plant Sci 66:119-126 https://doi.org/10.1016/0168-9452(90)90176-O
  15. Oh BK, U JK, Lee YI (2001) Improvement of Farfugium japonicum kitam. varieties induced by $\gamma$-ray irradiated mutation -variation of quantitative characters in $M_1$ generation of Farfugium japonicum Kitam. with various Doses. Kor J Hort Sci Technol 19:237
  16. Rugini E, Orlando R (1992) High efficiency shoot regeneration from calluses of in vitro shoot culture. J Hort Sci 67:577-582
  17. Seong NS, Park CH, Lee ST, Youn KB (1993) Callus induction and plant regeneration from axillary bud of Aconitum carmichaeli Debx. Kor J Breed 25:222-229
  18. Yoon KE, Kim HY (1993) Root and shoot regeneration from leaf disks of Brassica campestris ssp. pekinesis, B. rapa and Paphanus sativus. Kor J Plant Tiss Cult 20:167-170
  19. Yu CY, Cho HK, Ahn SD (1994) Effect of growth regulators and media on regeneration and plant growth in meristem culture of Aloe vera L. Kor J Breed 26:260-164

피인용 문헌

  1. Plant regeneration via somatic embryogenesis inFarfugium japonicum vol.34, pp.4, 2006, https://doi.org/10.1080/01140671.2006.9514425