Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of silver nitrate(AgNO3) and polyamines on shoot organogenesis and plant regeneration of Lycopersicon esculentum cultivar, Micro-Tom

질산은과 polyamines이 미니토마토, Micro-Tom 신초 기관발생과 식물체 재분화에 미치는 영향

  • Kim, Yong-Ho (Division of Biology, Hallym University) ;
  • Park, Cheol-Ho (College of Agriculture and Life Sciences, Kangwon National University) ;
  • Park, Sang-Un (Genepark Inc., Department of Biological Sciences, University of Calgary)
  • 김용호 (한림대학교 생명과학부) ;
  • 박철호 (강원대학교 농업생명과학대) ;
  • 박상언 (진파크 생명공학회사, 캘거리대학교 생물학과)
  • Published : 2002.03.01
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Abstract

The study was carried out to establish an efficient protocol for shoot organogenesis and plant regeneration from stem explant cultures of Lycopersicon esculentum cv. Micro-Tom. The regenerated shoots obtained from stem explant cultures on solid MS medium containing the different concentrations of BAP. The highest number of shoots (5.3) per explant and shoot growth (0.7 cm) was obtained on MS medium containing 4.0 mg/L BA. The additions of AgNO$_3$ and putrescine substantially improved the shoot regeneration frequency, at the optimal concentration of 7 mg/L and 50 mg/L respectively. The regenerated shoots (about 1 cm) were normal and could be easily rooted with 0.1 mg/L IBA treatment. The rooted plants were hardened and transferred to vermiculite with a 92% survival rate where they grew normally.

미니토마토, Micro-Tom (Lycopersicon esculentum cv. Micro-Tom)의 줄기 절편 배양으로부터 신초 기관발생을 통한 식물체 재분화 시스템을 확립하였다. 다른 농도의 BAP가 처리된 MS 고체 배지에서 신초 발생을 유도하였다. 신초 발생을 유도하기 위하여 cytokinin 종류와 농도별 처리에서는 4mg/L BAP 처리가 Micro-Tom 신초 기관분화를 위한 최적농도로 나타났으며, 줄기절편 당 평균 5.3개의 신초를 형성했고 0.7 cm 길이 신장을 보였다. 4 mg/L BAP이 처리된 MS 고체배지에 질산은 (7 mg/L)과 putrescine (50 mg/L) 처리로 신초기관발생을 향상시킬 수 있었다. 신초가 약 1 cm 길이로 생장하였을 때 기부를 절단하여 0.1 mg/L IBA이 처리된 MS 고형배치에 배양하여 뿌리를 유도하였으며, 발근된 식물체를 vermiculite에 옮겨 순화시킨 결과 92%의 생존율을 보였다.

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References

  1. Bajaj S, Rajam MV (1996) Polyamine accumulation and near loss of morphogenesis in long-term callus cultures of rice. Plant Physiology 112:1343-1348 https://doi.org/10.1104/pp.112.3.1343
  2. Basi HP, Sudha GS, Ravishankar GA (2000) Putrescine and silver nitrate influence shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow Local. J. Plant Growth Regul. 19:238-248
  3. Chi CL, Pua EC (1989). Ethylene inhibitor enhanced de novo shoot regeneration from cotyledons of B. campestris spp chinesis (Chinese cabbage) in Vitro. Plant Sci 64:243-250 https://doi.org/10.1016/0168-9452(89)90030-7
  4. Chraibi BKM, Latche A, Roustan J, Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt. Plant Cell Rep. 10:204-207
  5. Evans PT, Malmberg RL (1989) Do polyamines have a role in plant development? Ann Rev Plant Physiol Mol Biol 40:235-269 https://doi.org/10.1146/annurev.pp.40.060189.001315
  6. Fei S, Read PE, Riordan TP (2000) Improvement of embryogenic callus induction and shoot regeneration of Buffalograss by silver nitrate. Plant Cell Tiss Org Cult 60:197-203 https://doi.org/10.1023/A:1006468324616
  7. Fierer RP, Mignon G, Litvay JD (1984) Arginine decarboxylase and polyamine required for embryogenesis in wild carrot, Sciences 223:1433-1435 https://doi.org/10.1126/science.223.4643.1433
  8. Flores HE (1990) Polyamines and plant stress. In: Alscler RG, Cummy JR, Allen NS, editors. Sterss responses in plant adaptation and acclimatition mechanism. New York: Wiley Liss, Wiley Sons Inc., Pub pp 217-239
  9. Hatanaka T, Sawabe E, Azuma T, Uchida N, Yasuda T (1995) The role of ethylene in somatic embryogenesis from leaf discs of Coffea canephora. Plant Sci 107:199-204 https://doi.org/10.1016/0168-9452(95)04103-2
  10. Hyde CL, Phillips GC (1996) Silver nitrate promotes shoot development and plant regeneration of chile pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell Dev Biol 32: 72-80 https://doi.org/10.1007/BF02723036
  11. Kumar PP, Lakshmanan P, Thorpe TA (1998) Regulation of morphogenesis in plant tissue culture by ethylene. In Vitro Cell Dev Biol 34:94-103 https://doi.org/10.1007/BF02822771
  12. Meissner R, Jacoson Y, Melamed S, Levyatuv S, Shalev G, Ashri A, Elkind Y, Levy A (1997) A new model system for tomato genetics. Plant Journal 12:1465-1472 https://doi.org/10.1046/j.1365-313x.1997.12061465.x
  13. Mohiuddin AKM, Chowdhury MKU, Abdullah ZC, Napis S (1997) Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration. Plant Cell Tiss Org Cult 51:75-78 https://doi.org/10.1023/A:1005814514409
  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Ozean S, Barghchi M, Firek S, Draper J (1992) High frequency adventitious shoot regeneration from immature cotyledons of pea (Pisum sativum L). Plant Cell Rep 11:44-47
  16. Pua EC, Chi GL (1993) De novo shoot morphogenesis and plant growth of mustard (Brassica juncea) in vitro in relation to ethylene. Physiol Planta 88:467-474 https://doi.org/10.1111/j.1399-3054.1993.tb01361.x
  17. Pua EC, Teo SH, Loh CS (1996a) Interactive role of ethylene and polyamines on shoot regenerability of Chinese kale (Brassica alboglabra Bailey) in vitro. Journal of Plant Physiology 149:138-148 https://doi.org/10.1016/S0176-1617(96)80186-6
  18. Pua EC, Sim GE, Chi GL, Kong LF (1996b) Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyls explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro. Plant Cell Reports 15:685-690 https://doi.org/10.1007/BF00231925
  19. Purnhauser L, Medgyesy P, Czako M, Dix PJ, Marton L (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana pIumbaginifolia Viv. tissue cultures using the ethylene inhibitor Ag$NO_3$. Plant Cell Rep 6:1-4 https://doi.org/10.1007/BF00269725
  20. Scott JW, Harbaugh BK (1989) Micro-Toma miniature drarf tomato. Florida Agr Expt Sta Circ 370:1-6
  21. Shoeb F, Yadav JS, Bajaj S, Rajam MV (2001) Polyamines as biomarkers for plant regeneration capacity: Improvement of regeneration by modulation of polyamine metabolism in different genotypes of lndica rice. Plant Science 160:1229-1235 https://doi.org/10.1016/S0168-9452(01)00375-2
  22. Taylor PWJ, Ko HL, Fraser TA, Masel N, Adkins SW (1994) Effect of silver nitrate on sugarcane cell suspension growth, Protoplast isolation, ethylene production and shoot regeneration from cell suspension cultures. J Exp Bot 45:1163-1168 https://doi.org/10.1093/jxb/45.8.1163
  23. Walden R, Cordeiro A, Tiburcio AF (1997) Polyamines: small molecules triggering pathways in plant growth and development. Plant physiol 133:1009-1013
  24. Wehner TC (1999) Vegetable cultivar descriptions for North America list 25. HortSci 34:1004
  25. Xiaohan Y, Bo J, Yan Z, Ding M, Xuemei T (1995) Enhancement of direct shoot regeneration from internode segments of chry-santhemum by silver nitrate. Acta Hortic 404:68-73
  26. Yang SF (1985) Biosynthesis and action of ethylene. Hort Sci 20:41-45