Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Selection of Herbicide Tolerant Cell lines from $\gamma$-ray-Irradiated Cell Cultures in Rice (Oryza sativa L. cv. Ilpumbyeo)

  • Bae, Chang-Hyu (College of Education, Sunchon National University) ;
  • Lee, Young-Ill (Korea Atomic Energy Research Institute) ;
  • Lim, Yong-Pyo (College of Agricultural, Chungnam National University) ;
  • Seo, Yong-Won (College of Natural Resources, Korea University) ;
  • Lee, Do-Jin (College of Agricultural & Life Science, Sunchon National University) ;
  • Yang, Deuk-Chun (Institute of Korea of Korea Tabacco Research Institute) ;
  • Lee, Hyo-Yeon (College of Agricultural & Life Science, Sunchon National University)
  • 발행 : 2002.09.01
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초록

Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.

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참고문헌

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