Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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A Unique Strategy for Recovering Recombinant Proteins from Molecular Farming: Affinity Couture on Engineered Oilbodies

  • Seon, Jeong-Hoon (SemBioSys Genetics Inc., Canada) ;
  • J.Steven Szarka (SemBioSys Genetics Inc., Canada) ;
  • Maurice M. Moloney (SemBioSys Genetics Inc., Canada)
  • Published : 2002.09.01
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Abstract

Molecular faming has the potential to provide large amounts of recombinant protein for use in diagnostics and as therapeutics. Various strategies have been developed to enhance the expression level, stability, and native folding of recombinant proteins produced in plants. Few investigations into the subcellular distribution of recombinant proteins within plant cells have been published despite the potential to increase the expression level and impact the purification process. This review article discusses the current strategies used for targeting recombinant proteins to various subcellular locations and the advantages of targeting to seed oil bodies for molecular farming applications. Specifically, the affinity capture of antibodies using recombinant oilbodies is discussed.

Keywords

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