Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Parentage Identification of 'Daebong' Grape (Vitis spp.) Using RAPD Analysis

  • Kim, Seung-Heui (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Jeong, Jae-Hun (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Kim, Seon-Kyu (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
  • Published : 2002.06.01
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Abstract

The RAPD data were used to assess genetic similarity among f grape cultivars. Of the 100 random primers tested on genomic DNA, 10 primers could be selected for Benetic analysis, and the selected primers generated a total of 115 distinct amplification fragments. A similarity matrix was constructed on the basis of the presence or absence of bands. The 7 grape cultivars analyzed with UPGMA were clustered into two groups of A and B. The similarity coefficient value of cultivars was high. The mean similarity index for all pairwise comparisons was 0.851, and ranged from 0.714 ('Rosaki' and 'Black Olympia') to 0.988 ('Kyoho' and 'Daebong'). After due consideration of differences in cultural and morphological characteristics of these two theoretically identical cultivars, it could be deduced that 'Daebong' is a bud sport of 'Kyoho' cultivar.

Keywords

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