Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of GA3 and Charcoal on Plant Regeneration from Somatic Embryos of Acanthopanax sessiliflorus

오가피(Acanthopanax sessiliflorus)의 체세포배로부터 식물체 재생에 미치는 GAa3와 Charcoal의 영향

  • Lee, Kang-Seop (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Choi, Yong-Eui (Korea ginseng Institute, Chung-Ang University) ;
  • Sim, Ock-Kyeong (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Joo, Sun-Ah (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Shin, Jeong-Sun (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Jeong, Jae-Hun (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Kim, Young-Shin (Panax-Bio Institute, Center for Biotechnology and Bioventure) ;
  • Kim, Ee-Yup (Panax-Bio Institute, Center for Biotechnology and Bioventure)
  • Published : 2002.12.01
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Abstract

To establish the optimum condition for plant regeneration from somatic embryos of Acanthopanax sessiliflorus Rupr. et Maxim, a medicinal plant, somatic embryos were induced from zygotic embryo-derived embryogenic callus in hormoen-free MS medium. To induce plantlet conversion, cotyledonary somatic embryos were cultured on MS solid medium with GA$_3$at various concentrations (0~10 mg/L) for three weeks. Plantlets were transferred to 1/3 MS solid medium with 0.5% charcoal for 7 weeks. Stem length was increased proportionally to the concentration and treatment period of GA$_3$. Also, the highest leaf width (8.9 mm) and leaf number (2.84) of plantlet were obtained when plantlets were converted on 5,10 mg/L GA$_3$pretreatments, respectively. The highest plant conversion frequency (66.7%) was obtained when the somatic embryos were cultured on medium containing 5 mg/L GA$_3$ for 3 weeks and then were transferred to 1/3 MS medium with 0.5% charcoal. The highest survival rate of soil transfer was 90% when plantlets were regenerated on medium with 5 mg/L GA$_3$ for 3 weeks and then transferred to plastic pots containing vermiculite and sand mixture for 4 weeks.

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References

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