Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Molecular typing and detection of enterotoxin by multiplex PCR of Staphylococcus aureus isolated from bovine mastitis

유방염 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출

  • Kim, Sin (Northern Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Hong, Hyon-Pyo (Dept of Biology, Andong National University) ;
  • Kim, Sang-Yun (Dept of Biology, Andong National University) ;
  • Kwon, Heon-Il (Dept of Biology, Andong National University) ;
  • Lee, Hee-Moo (Dept of Biology, Andong National University)
  • 김신 (경상북도가축위생시험소 북부지소) ;
  • 홍현표 (안동대학교 자연과학대학) ;
  • 김상윤 (안동대학교 자연과학대학) ;
  • 권헌일 (안동대학교 자연과학대학) ;
  • 이희무 (안동대학교 자연과학대학)
  • Published : 2002.09.01
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Abstract

Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.

Keywords

References

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