한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제25권3호
  • /
  • Pages.221-227
  • /
  • 2002
  • /
  • 3022-7372(pISSN)
  • /
  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지

LC/MS를 이용한 축산물 중 잔류 마크로라이드계 항생물질 분석법 연구

Study on analytical method of residual macrolide antibiotics in livestock products by LC/MS

  • 황래홍 (서울특별시보건환경연구원) ;
  • 윤은선 (서울특별시보건환경연구원) ;
  • 김연주 (서울특별시보건환경연구원) ;
  • 김동언 (서울특별시보건환경연구원) ;
  • 양윤모 (서울특별시보건환경연구원) ;
  • 이정학 (서울특별시보건환경연구원) ;
  • 이병동 (서울특별시보건환경연구원)
  • 발행 : 2002.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study was carried out to confirm analytical method of residual macrolides in livestock products by LC/MS. 1. Macrolides were analyzed by LC/MS on XTerra C$\sub$18/ column with 0.1% TFA(trifluoroacetic acid)-methanol in a gradient mode as mobile phase, and that were identified by positive chemical ionization with selective ion monitoring at 50～1000 mass range. 2. Residual macrolides were extracted from tissue with acetonitrile, and the extract is purified with a Sep-pak C$\sub$18/ cartridge, and elute macrolides with 0.1M methanolic ammonium acetate. 3. The procedure confirms the presence of each macrolide at 50$\mu\textrm{g}$/kg in spiked sample.

키워드

참고문헌

  1. Delepine B, Hurtaud-Pessel D, Sanders P. 1996. Multiresidue method for confirmation of macrolide antibiotics in bovine muscle by liquid chromatography/mass spectrometry. JAOAC Int 79 : 397-404
  2. Juhel-Gaugain M, Anger B. 1999. Multiresidue chromatographic method for the determination of macrolide residues in muscle by high-performance liquid chromatography with UV detection. JAOAC Int 82 : 1046-1052
  3. Chan W, Gerhardt GC, Salisbury CDC. 1994. Determination of tylosin and tilmicosin residues in animal tissues by reversed-phase liquid chromatography. JAOAC int 77 : 331-333
  4. Boison JO, Salisbury CDC, Waynechan, et al. 1991. Determination of penicillin G residues in edible animal tissues by liquid chromatography. JAOAC Int 74 : 497-501
  5. Meetschen U, Petz M. 1990. Capillary gas chromatographic method for determination of benzylpenicillin and other beta lactam antibiotics in milk. JAOAC Int 73 : 373-378
  6. Zomer E, Quintana J, Saul S, et al. 1995. A method for identification and quantitation of $\beta$-lactams in milk by liquid chromatography with microbial receptor assay. JAOAC Int 78 : 1165-1172
  7. Cutting JH, Kiessling WM, Bond FL, et al. 1995. Agarose gel electrophoretic detection of six $\beta$-lactam antibiotic residues in milk. JAOAC Int 78: 663-667
  8. Rogers ME, Adlard MW. Saunders G, et al. 1984. Derivatization techniques for high performance liquid chromatographic analysis of $\beta$-lactams. J Chromatogr 297: 385-391 https://doi.org/10.1016/S0021-9673(01)89059-2
  9. Boison JOK, Keng LJY, MacNel JD. 1994. Analysis of penicillin G in milk by liquid chromatography. JAOAC Int 77 : 565-569
  10. Moats WA. 1994. Determination of ampicillin and amoxicillin in milk with an automated liquid chromatographic cleanup. JAOAC Int 77 : 41-45
  11. Moats WA, Harik-Khan R. 1995. Liquid chromatographic determination of $\beta$-lactams antibiotics in milk: A multiresidue approach. JAOAC Int 78 : 49-54
  12. Moats WA. 1992. Determination of cloxacillin and penicillin V in milk using an automated liquid chromatographic cleanup. JAOAC Int 75: 257-260
  13. Moats WA. 1997. Advances in determination of antibiotic residues. JAOAC Int 80:1-4