Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Purification and Characterization of Extracellular Protease form Psychrotrophic Antarctic Bacteria

남극에서 분리한 저온성 세균 유래 단백질 분해 효소

  • 조기웅 (한국해양연구원 해양천연물 연구실) ;
  • 방지헌 (한국해양연구원 해양천연물 연구실) ;
  • 홍혜원 (한국해양연구원 해양천연물 연구실) ;
  • 박승일 (한국해양연구원 해양천연물 연구실) ;
  • 이윤호 (한국해양연구원 해양천연물 연구실)
  • Published : 2002.12.01
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Abstract

A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.

저온에서 최적 생육을 보이는 저온성 균주를 남극해양에서 분리하여 생화학적 특성 및 165 rRNA 염기서열로부터 Shewanella sp.에 속하는 균주로 동정하고 Shewanella sp. L93으로명명하였다. 본 균주에서 생산되는 저온성 세포외 단백질 분해 효소(extracellular protease)를 ammonium sulfate precipitation, High-Q column chromatography, 일차 gel permeation chromatography, BioScale Q2 ion exchange chromatography 및 gel permeation chromatography를 통하여 purification fold 19.3, yield 0.7 %로 정제하였고 그 특성을 조사하였다.

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References

  1. 현대의 생명공학과 생물산업 효소를 이용한 바이오산업의 전망 오평수
  2. J. Aquat. Anim. Health v.6 Electrophoretic detection of proteases from selected strains of Flexibacter psychrophilus and assessment of their variability Bertolini, J.M;H. Wakabayashi;V.G. Watral;M.J. Whipple;J.S. Rohovec
  3. Can. J. Microbiol. v.21 Partial purification and characterization of lipase of a facultative psychrophilic bacterium(Acinetobacter O16) Brenil, C.;J. Kushner
  4. Appl. Environ. Microbiol. v.64 A cold-adapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization Choo, D.;T. Kurihara;T. Suzuki;K. Soda;N. Esaki
  5. FEMS Microbiol. Lett. v.66 Lipases from psychrotrophic antarctic bacteria Feller, G.;M. Thiry;J. L. Arpigny;M. Mergeay;C. Gerday
  6. Advances in Biochemical Engineering and Biotechnology Microbial proteinase Fiechter, A.
  7. Aquaculture v.13 Columnaris disease: recent advances in research Griffin, B.R.
  8. J. Appl. Microbiol. v.83 Pathogenicity of atypical Aeromonas salmonicida in Atlantic salmon compared with protease production Gunnlaugsdottir, B.;B.K. Gudmundsdottir
  9. Extremophiles Psychrophiles Hamamoto, T.;N.J. Russell;K. Horikoshi(ed.);W.D. Grant(ed.)
  10. Infect. Immun. v.50 Production and partial characterization of an elastolytic protease of Vibrio vulnificus Kothary, M.H.;A.S. Kreger
  11. Nature(London) v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U.K.
  12. Infect. Immun. v.56 Tn5-induced protease-deficient strains of Aeromonas hydrophila with reduced virulence for fish Leung, K.Y.;R.M.W. Stevenson
  13. Infect. Immun. v.58 Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum Norqvist, A.;B. Norrman;H. Wolf-Watz