Effects of Acidification on the Species Composition and the Changes of Extracelluar Enzymes of Heterotrophic Bacterial Community

수계종속 영양세균 군집의 종조성 및 세포외 효소의 변화에 미치는 산성화의 영향

  • Choi, Yong-Keel (Department of Life Science, Hanyang University) ;
  • Han, Myung-Soo (Department of Life Science, Hanyang University) ;
  • Kim, Sewha (Department of Life Science, Yong-In University) ;
  • Lee, Kyung (Department of Biology, The Catholic University of Korea) ;
  • Yoo, Kwang-Il (Department of Life Science, Hanyang University)
  • 최영길 (한양대학교 자연과학대학 생명과학과) ;
  • 한명수 (한양대학교 자연과학대학 생명과학과) ;
  • 김세화 (용인대학교 생명과학과) ;
  • 이경 (가톨릭대학교 생물학부) ;
  • 유광일 (한양대학교 자연과학대학 생명과학과)
  • Published : 2002.03.01

Abstract

In an artificial pH-gradient batch culture system, the author analyzed the effects of acidification on the species composition of heterotrophic bacteria. As the result of this study, it was found that the numbers of total bacteria were not affected by acidification and that the population size of heterotrophic bacteria decreased as pH became lower. The heterotrophic bacteria isolated from all of the pH gradient were 12 genera and 22 species, and among them, gram negative and gram positive bacteria were 04% and 30%, respectively. As pH decreased, the distribution rate of gram negative bacteria increased while that of gram positive bacteria decreased. Regarding to distribution rate of genuses in each pH gradient, 13 genuses appeared at pH 7 while only 5 genuses appeared at pH 3, which means that the diversity of genera decrease as pH decreased. The activities of extracellular enzyme showed the ranges of $0.008-0.292\;\mu{M}\ell^{-1}\;hr^{-1}$ in bioreactor system. The enzymatic activities decreased rapidly below pH 5 and then sustained 5-38% at the lower pH values.

인위적으로 pH 구배가 조성된 회분배양 시스템 (batch culture system)내에서 산성화에 따른 종속영양 세균 군집의 종조성 변화를 분석하였다. 총세균의 개체수는 산성화에 영향을 받지 않았으며, 종속영양세균 군집의 크기는 pH가 낮아질수록 감소하였다. 전 pH구간에서 분리된 종속영양 세균들은 모두 12속 22종으로 나타났으며, 이중 그람음성 세균은 64%,그람양성 세균은 36%의 비율로 분포하였다. pH가 낮아짐에 따라 그람양성 세균의 분포 비율은 감소하는 반면, 그람음성 세균의 분포 비율은 증가하는 양상을 보였다. 각 pH 구간별 속의 분포 비율은 pH 7에서는 12개 속이 출현하였으나, pH 3에서는 5개 속만 출현하여 pH가 낮아짐에 따라 속의 다양성이 감소하는 양상을 나타내였다. 세포외 효소의 활성도는 $0.008-0.292\;\mu{M}\ell^{-1}\;hr^{-1}$의 범주로 나타났고, 산성화에 따라 세포외 효소의 활성도는 급격히 감소하여 pH 5이하에서는 5-38%의 낮은 활성을 나타내었다.

Keywords

References

  1. 한국대기보전학회지 v.12 서울지역 강수중 이온 성분 분석자료의 해석 강공언;이주희;김희강
  2. 한국대기보전학회지 v.13 서울지역 강수 산성도의 장기적인 경향분석 강공언;임재현;김희강
  3. 국립환경연구원보 v.12 대기오염물질의 장거리 이동과 산벙비 강하에 관한 연구Ⅱ 김양균;강인구;이민희;나진균;이석조;한의정;한진석;신찬기;김정수;유승도;홍율기;정준화;이재인;박태술;권오영;강은희
  4. 한국생태학회지 v.20 no.1 산성토양 개량제 처리에 따른 식물의 생장반응과 토양성질의 변화 문형태;박병규;김준호
  5. 한국수질보전학회지 v.12 산성우에 의한 토양내의 이온 거동에 관한 연구 송승완;최찬섭;정형근;권영식
  6. 한국육수학회지 v.29 회분배양 시스템에서 산성화에 따른 세균군집의 변화 안영범;조흥범;최영길
  7. 미생물과 사업 v.22 서천 연안 퇴적토에서 종속영양 세균의 분포와 세포의 효소 활성간의 관계 최강국;이건형
  8. Kor. J. Microbiol v.33 Effects of hydrogen ion on aquatic population of microbes in Korea Ahn YB;HB. Cho;YK Choi
  9. American Public Health Association(18th ed.) Standard methods for the examination of water and coast water American Public Health Association;American Water Works Association;Water Environment Federation
  10. Advances in microbial Ecology v.7 Diversity of microbial communities Atlas RM
  11. In Workshop for Environmental Science and Technology, The Korean Federation of Science and Technology Societies An International Collaborative Projoct on Acid Rain in Asia Carmichael GR;M Amann;A Azimi;SC Bhattacharya;RM Shrestha;M Chadwick;J Kluylenstierna;S Cinderby;W Foell;C Green;JP Hettelingh;L hordijk;J Shah;MP Singh;D Zhao
  12. Limmnol. Oceanon v.34 Characterization and significance of β-glumsidase activity in lake water Chrost RJ https://doi.org/10.4319/lo.1989.34.4.0660
  13. J. Bacteriol v.101 Aerobic heterotrophic bacteria indigenous to pH 2.8 acid mine water; predominant slime-producing bacteria in acid streams Dugan PR;CB MacMillan;RM Pfister
  14. In JM Lynch and NJ Poole (ed.) Microbial Ecology: A conceptual Approach The aquatic environment Fletcher M
  15. Appl. Environ. Microbiol v.33 use of nuclepore filters for counting bacteria by fluorescence microscopy Hobbie JE;RJ Daley;S Jasper
  16. Mar. Ecol Prog. ser. v.11 Significance of exoenzymatic activities in the ecology of backish water : Measurements by means of methylumbelliferyl-substrates Hoppe HG https://doi.org/10.3354/meps011299
  17. Acid rain and acid waters Howells G.
  18. In M Fletcher and TRG Gray (ed). Ecology of microbi8ol communities Diversity in freshwater microbiology Jones JC
  19. Proceedings. '96 Symposium of Korean Society of Limnology Trophic state of reservoirs in Korea Kim BC;JH Park;GS Hwang;KS Choi
  20. Microb. Ecol v.14 The communjty structure of sessile heterotrophic bacteria stressed by acid mine drainage Mills AL;LM Mallory https://doi.org/10.1007/BF02012942
  21. J. Appl. Bacteriol v.75 Effects of pH and temperature on heterotrophic bacteria in acidified and non-acidified lochs Rattray J;NA Logan https://doi.org/10.1111/j.1365-2672.1993.tb02778.x
  22. Microbial ecology of a brackish water environment Rheinherimer G
  23. Environ. Monit. Assess v.12 Losses of biota from American aquatic communities due to acid rain Schindler DW;SE Kasian;RH Hesskin
  24. Biol. Fertil. Soils v.17 Enzymic analysis of microbial pattern and Process Sinsabaugh RL https://doi.org/10.1007/BF00418675
  25. Appl. Environ. Microbiol. v.48 Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water Somville M
  26. Microb. Ecol v.9 Change in water and sedi-ment bacterial community structure in a lake receiving acid mine drainage Wassel RA;AL Milk https://doi.org/10.1007/BF02015128