한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제22권1호
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  • Pages.51-58
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  • 2002
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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배추로부터 광계 I의 PSI-H Subunit Homolog의 클로닝 및 분자생물학적 특성 연구

Molecular Cloning and Characterization of a cDNA for the PSI-H Subunit Homolog of Photosystem I in Chinese Cabbage

  • 차준영 (경상대학교 식물분자생물학 및 유전자조작연구소) ;
  • 최영진 (경상대학교 식물분자생물학 및 유전자조작연구소) ;
  • 이효신 (임업연구원 생물공학과) ;
  • 김기용 (축산기술연구소) ;
  • 박근제 (축산기술연구소) ;
  • 조진기 (경북대학교 동물공학과) ;
  • 손대영 (경상대학교 식물분자생물학 및 유전자조작연구소)
  • 발행 : 2002.03.01
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초록

식물의 광합성에 관여하는 광계 I의 protein subunit들의 연구는 최근까지도 극히 미약한 실정이며, 각각의 subunit들의 특성 또한 일부만이 밝혀져 있다. 본 연구진은 배추의 cDNA library로부터 식물에만 존재하는 subunit 중의 하나인, PSI-H subunit을 암호화하는 유전자인 bpsaH를 분리하였다. 이 유전자는 총 633 bp의 염기로 구성되어 있으며, 염기서열로부터 추정되는 분자량은 약 15,400이었고 등전점은 9.91이었다. 배추 PSI-H subunit의 아미노산 서열을 다른 식물체 유래의 단백질들과 비교분석한 결과, 시금치의 PSI-H와 가장 높은 유사성 (79.3%)을 나타내었다. 또한 bpsaH의 조직 특이적 발현 양상을 조사한 결과, 광합성 조직인 잎에서는 강하게 발현된 반면 꽃봉우리에서는 약하게 발현되었으며, 비광합성 조직인 뿌리에서는 전혀 발현되지 않았다.

PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.

키워드

참고문헌

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